| 牛精子全蛋白二维电泳等电聚焦程序优化及染色方法的比较 |
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| 引用本文： | 陈龙,朱化彬,王栋,郝海生,杜卫华,沙里金.牛精子全蛋白二维电泳等电聚焦程序优化及染色方法的比较[J].中国畜牧兽医,2008,35(12):43-46. |
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| 作者姓名： | 陈龙 朱化彬 王栋 郝海生 杜卫华 沙里金 |
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| 作者单位： | 1.甘肃农业大学动物科学技术学院，兰州 730070；2.中国农业科学院北京畜牧兽医研究所，北京 100193；3.北京奶牛中心，北京 100085 |
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| 基金项目： | 国家自然科学基金，国家高技术研究发展计划(863计划)，科技支撑项目 |
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| 摘 要： | 以牛精子全蛋白为试验材料进行二维电泳试验研究，优化和改进了一向等电聚焦参数，比较了不同的染色方法，并运用Image Master 6.0软件分析了二维电泳图谱。结果表明，采用24 cm，pH3～10线性IPG胶条进行牛精子全蛋白二维电泳，等电聚焦80000 Vh和结合硝酸银染色方法可得到较多蛋白点和较高分辨率的二维电泳图谱。
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| 关 键 词： | 牛 精子全蛋白 二维电泳 |
Optimizing the IEF Protocols and Comparing the Staining Methods of the 2D Electrophoresis for the Global Proteins of Bovine Spermatozoa |
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| CHEN Long,ZHU Huab-in,WANG Dong,HAO Hai-sheng,DU Wei-hua,SHA Li-jin.Optimizing the IEF Protocols and Comparing the Staining Methods of the 2D Electrophoresis for the Global Proteins of Bovine Spermatozoa[J].China Animal Husbandry & Veterinary Medicine,2008,35(12):43-46. |
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| Authors: | CHEN Long ZHU Huab-in WANG Dong HAO Hai-sheng DU Wei-hua SHA Li-jin |
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| Institution: | 1.Faculty of Animal Science & Technology, Gansu Agricultural University,Lanzhou 730070, China;2.Institute of Animal Science, Chinese Academy of Agricultural Sciences,Beijing 100193, China;3.Beijing Dairy Cattle Center,Beijing 100085, China |
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| Abstract: | The IEF protocols of the two dimensional gel electrophoresis for bovine spermatozoa proteins was optimizied, the staining methods were comparied and the 2 D image was analyzied with the software of Image Master 6.0. The result indicated that the better stability and reproducibility of 2 D image was acquired by using the 24cm (pH3～10) Linar strips, focusing for 80000Vh and combining with the silver staining methods. |
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| Keywords: | bovine spermatozoa proteins two-dimensional gel electrophoresis |
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