| 拟南芥Atsuc2突变体愈伤组织的诱导及再生体系的建立 |
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| 引用本文： | 崔震海,李晓宇,张立军,汪澈,阮燕晔,樊金娟.拟南芥Atsuc2突变体愈伤组织的诱导及再生体系的建立[J].河南农业科学,2009(5). |
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| 作者姓名： | 崔震海 李晓宇 张立军 汪澈 阮燕晔 樊金娟 |
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| 作者单位： | 1. 沈阳农业大学,生物技术学院,辽宁,沈阳,110161
2. 沈阳农业大学,生物技术学院,辽宁,沈阳,110161;辽宁省杨树研究所,辽宁,盖州,115200 |
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| 基金项目： | 国家自然科学基金项目，沈阳农业大学青年教师科研基金项目，辽宁省教育厅科研基金项目，辽宁省教育厅高校科研计划项目 |
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| 摘 要： | 拟南芥蔗糖转运蛋白基因AtSUC2是蔗糖转运的主效基因,其缺失突变体在有糖培养基上可以生长和开花,但得不到有活力的种子,因此难以获得足够的实验材料对AtSUC2的功能进行深入研究。针对这一问题,以拟南芥Atsuc2纯合突变体的不同外植体为材料进行愈伤组织诱导及再生体系建立的研究。结果表明:诱导愈伤组织的最佳外植体为具柄叶切片,其在B5+3.0 mg/L2,4-D+0.5 mg/L KT的培养基上愈伤组织出现的时间最早,质量最好;产生的愈伤组织在配方为MS+2.0 mg/L 6-BA+0.1 mg/L IBA的生芽培养基上被诱导生芽,分化率可以达到86.7%;在配方为MS+1.0 mg/L IBA+0.1 mg/L 6-BA的生根培养基上丛生芽生根率为85.3%。
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| 关 键 词： | 拟南芥 蔗糖转运蛋白 Atsuc2 愈伤组织 再生体系 |
Callus Induction and Plantlet Regeneration System from Atsuc2 Mutant of Arabidopsis thaliana |
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| CUI Zhen-hai,LI Xiao-yu,ZHANG Li-jun,WANG Che,RUAN Yan-ye,FAN Jin-juan.Callus Induction and Plantlet Regeneration System from Atsuc2 Mutant of Arabidopsis thaliana[J].Journal of Henan Agricultural Sciences,2009(5). |
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| Authors: | CUI Zhen-hai LI Xiao-yu ZHANG Li-jun WANG Che RUAN Yan-ye FAN Jin-juan |
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| Institution: | 1.School of Biotechnology;Shenyang Agricultural University;Shenyang 110161;China;2.Liaoning Provincial Institute of Poplar;Gaizhou 115200;China |
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| Abstract: | Sucose transport protein gene AtSUC2 of Arabidopsis thaliana is a major gene in its gene family,its homozygous mutant(Atsuc2) could grow and flower on medium with sucrose,but producing seeds without viability.Therefore,it is difficult to obtain enough Atsuc2 plants to explore the role of this gene.This paper investigated the callus induction and establishment of plantlet regeneration system with different explants of Atsuc2.The results showed that the leaf discs with a petiole was the most suitable explants... |
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| Keywords: | Atsuc2 |
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