| 中国红皮砂梨品种的SSR标记分析 |
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| 引用本文： | 张东,舒群,滕元文,仇明华,鲍露,胡红菊.中国红皮砂梨品种的SSR标记分析[J].园艺学报,2007,34(1):47-52. |
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| 作者姓名： | 张东 舒群 滕元文 仇明华 鲍露 胡红菊 |
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| 作者单位： | ( 浙江大学园艺系, 农业部园艺植物生长发育与生物技术重点开放实验室, 杭州310029; 云南省农业科学院园艺研究所, 昆明650205; 湖北省农业科学院果树茶叶研究所, 武汉430209) |
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| 基金项目： | 国家自然科学基金;云南省科技厅资助项目 |
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| 摘 要： | 采用SSR (Simp le sequence repeat) 标记技术对29个砂梨品种或类型(主要为红皮型) 做了鉴定, 并对其亲缘关系进行了分析。6对SSR引物(BGA35、KU10、BGT23b、NH004a、NH011b和NH015a)均扩增出了较多的等位基因。NH004a位点的等位基因数、有效等位基因、杂合度和香农多样性指数都较高,显示了良好的品种鉴定能力。除3对品种或类型可能为同物异名或芽变类型无法区分开外, 6对引物组合可以成功地鉴定其它品种。聚类分析可以将29个砂梨品种(类型) 明显分成4个组。第Ⅰ组全部来自云南, 其中包括2个绿皮砂梨品种, 其余为红皮砂梨, 说明该组内红皮砂梨的祖先可能与这些绿皮砂梨亲缘关系很近。在第Ⅱ、Ⅲ组中, 来自四川会理的红梨品种和云南的红梨品种交叉组合, 可能是两地间品种交流的历史反映。第Ⅳ组中来自四川会理的‘香酥梨’、‘栽秧梨’, 原产云南弥渡的‘弥渡香酥梨’以及原产云南丽江的‘长水火把梨’相互间及与其它梨的亲缘关系均较远。分布在云南各地的火把梨可能有不同的起源。
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| 关 键 词： | 砂梨 SSR 品种鉴定 亲缘关系 |
| 文章编号： | 0513-353X（2007）01-0047-06 |
| 收稿时间： | 2006-04-01 |
| 修稿时间： | 2006-04-012006-11-21 |
Simple Sequence Repeat Analysis on Genetic Assessment of Chinese Red Skinned Sand Pear Cultivars |
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| ZHANG Dong,SHU Qun,TENG Yuan-wen,QIU Ming-hua,BAO Lu,HU Hong-ju.Simple Sequence Repeat Analysis on Genetic Assessment of Chinese Red Skinned Sand Pear Cultivars[J].Acta Horticulturae Sinica,2007,34(1):47-52. |
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| Authors: | ZHANG Dong SHU Qun TENG Yuan-wen QIU Ming-hua BAO Lu HU Hong-ju |
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| Institution: | (;1. Departm ent of Horticulture, Zhejiang University, the State Agricultural M inistry Laboratory of Horticultural Plant Growth,Development & Biotechnology, Hangzhou 310029, China;2. Institute of Horticulture, Yunnan Academy of Agricultural Sciences,Kunming 650205, China;3. Institute of Friut and Tea, Hubei Academy of Agricultural Sciences, Wuhan 430209, China) |
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| Abstract: | A total of 29 sand pear germp lasm (mainly red skinned) native to southwest of China weresubjected to simp le sequence repeat ( SSR) analysis. Six pairs of SSR p rimers (BGA35, KU10, BGT23b,NH004a, NH011b and NH015a) could generate a large number of alleles. The p rimer NH004a, which p roduced the highest allele numbers and the most effective alleles, and high heterozygosity and Shannon information index, showed the best identification power. Using six pairs of SSR p rimers, all accessions or types couldbe distinguished excep t for three pairs of cultivars, which might belong to the synonymy cases or bud mutants.Twenty nine pear accessions could be divided into fourmajor group s obviously based on the UPGMA cluster analysis. Group Ⅰ consisted of cultivars all native to Yunnan p rovince, including two green and nine redskinned sand pears, inferring a near genetic relationship between the red and green skinned pears. In groupsⅡand Ⅲ, the red skinned pears from Huili County of Sichuan Province and from Yunnan Province mingled together, which might suggest cultivarmovement from Yuannan to Sichuan. In group Ⅳ, not onlywere Xiangsuli and Zaiyangli from Huili County of Sichuan Province, Midu Xiangsuli and Changshui Huobali native to Yunnan Province not closely clustered, but also were far from other groups. Huobali pear types distributed in Yunnan and Sichuan p rovincesmight have different origins. The results above indicates large genetic diversity ofgermp lasm resources of red skinned sand pear cultivars in China |
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| Keywords: | SSR |
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