| 小麦新品种百农矮抗58及其亲本矮秆基因的检测 |
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| 引用本文： | 王刚,胡铁柱,李小军,董娜,冯素伟,李淦,张立琳,茹振钢.小麦新品种百农矮抗58及其亲本矮秆基因的检测[J].河南农业科学,2012,41(9):22-25. |
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| 作者姓名： | 王刚 胡铁柱 李小军 董娜 冯素伟 李淦 张立琳 茹振钢 |
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| 作者单位： | 河南科技学院小麦研究中心,河南新乡,453003 |
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| 基金项目： | 国家“十二五”科技支撑计划项目(2011BAD07B02);河南省科技攻关计划项目(102102110032) |
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| 摘 要： | 为探索百农矮抗58小麦所含的矮秆基因及其来源,应用赤霉素反应和分子标记检测了百农矮抗58及其亲本周麦11、豫麦49号、郑州8960的矮秆基因。结果表明:郑州8960为赤霉素敏感型,其他3个品种对赤霉素不敏感;分子标记检测显示,矮抗58、豫麦49号、郑州8960携带Rht-D1b基因,周麦11携带Rht-B1b基因,4个品种均没有扩增出Rht8基因的192bp标准带。
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| 关 键 词： | 小麦 百农矮抗58 矮秆基因 分子标记 |
Detection of Dwarfing Genes in Wheat Variety AK58 and Its Parents |
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| WANG Gang
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HU Tie-zhu
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LI Xiao-jun
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DONG Na
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FENG Su-wei
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LI Gan
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ZHANG Li-lin
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RU Zhen-gang.Detection of Dwarfing Genes in Wheat Variety AK58 and Its Parents[J].Journal of Henan Agricultural Sciences,2012,41(9):22-25. |
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| Authors: | WANG Gang HU Tie-zhu LI Xiao-jun DONG Na FENG Su-wei LI Gan ZHANG Li-lin RU Zhen-gang |
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| Institution: | (Wheat Research Center,Henan Institute of Science and Technology,Xinxiang 453003,China) |
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| Abstract: | In order to explore the source of dwarfing genes in wheat variety AK58,AK58 and its parents Zhoumai 11,Yumai 49 and Zhengzhou 8960 were analyzed by means of gibberellic acid(GA3) reaction and STS markers.The results indicated that Zhengzhou 8960 was sensitive to GA3 and other cultivars were insensitive.Molecular marker confirmed that Rht-D1b was detected in AK58,Yumai 49 and Zhengzhou 8960,Rht-B1b was present in Zhoumai 11,while no Rht8 gene was found in above 4 varieties. |
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| Keywords: | wheat AK58 dwarfing genes molecular marker |
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