| 过氧化物酶用于山楂嫁接亲和性早期鉴定研究 |
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| 引用本文： | 王威,彭金根,刘燕,王凤洲.过氧化物酶用于山楂嫁接亲和性早期鉴定研究[J].河南农业科学,2012,41(6):119-123. |
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| 作者姓名： | 王威 彭金根 刘燕 王凤洲 |
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| 作者单位： | 北京林业大学园林学院,国家花卉工程技术研究中心,北京100083 |
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| 摘 要： | 木本植物嫁接体生长多年才表现出不亲和,为实现猩红英国山楂不同嫁接组合砧穗亲和性的早期鉴定,研究了猩红英国山楂及其砧木过氧化物酶(POD)的同工酶谱带,并对嫁接后各组合砧木和接穗的POD活性进行了动态测定。结果表明,砧穗POD同工酶谱带相同值的大小可以反映嫁接亲和性的强弱。嫁接后,砧穗POD活性差异小且逐渐变小的猩红英国山楂/单子山楂组合亲和性强,砧穗POD活性差异大且逐渐增大的猩红英国山楂/辽宁山楂组合亲和性差。因而认为,砧穗的POD活性可以作为山楂嫁接亲和性的早期鉴定指标。
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| 关 键 词： | 过氧化物酶 同工酶 嫁接亲和性 早期鉴定 山楂 |
Early Detection of Graft-compatibility in Crataegus L.Using POD Analysis |
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| WANG Wei
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PENG Jin-gen
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LIU Yan
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WANG Feng-zhou.Early Detection of Graft-compatibility in Crataegus L.Using POD Analysis[J].Journal of Henan Agricultural Sciences,2012,41(6):119-123. |
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| Authors: | WANG Wei PENG Jin-gen LIU Yan WANG Feng-zhou |
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| Institution: | (College of Landscape Architecture,Beijing Forestry University,National Engineering Research Center for Floriculture,Beijing 100083,China) |
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| Abstract: | Experiments were conducted to analyze the peroxidase isoenzymes of the ornamental hawthorn cultivar(Crataegus laevigata ’Paul’s Scarlet’) with three kinds of hawthorn rootstocks(C.pinatifida,C.sanguinea,C.monogyna),and to measure the POD activity of scion and rootstock bark after grafting.The results were as follows:the same count value of POD isoenzyme of scion and rootstock was related to graft-compatibility,the former was bigger and the latter was stronger.As growth of the graft union,the difference value of POD activity between rootstock and scion became smaller from Clps/Cm of which the compatibility was stonger,and from Clps/Cs,the difference value became bigger,while the compatibility was weaker.So the POD activity at graft unions could be used as a method for early detection of graft-compatibility. |
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| Keywords: | POD isoenzyme graft-compatibility early detection Crataegus L |
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