Typing of Yersinia Enterocolitica Isolates by ITS profiling, REP- and ERIC-PCR |
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| Authors: | Wojciech Ł Staroniewicz Z Jakubczak A Ugorski M |
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| Institution: | Department of Biochemistry, Pharmacology and Toxicology, Faculty of Veterinary Medicine, Agriculture University Norwid St. 31, 50-375 Wroclaw, Poland. |
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| Abstract: | Thirty-five Yersinia enterocolitica strains isolated from humans, pigs and foxes were analysed by genotyping including intergenic transcribed sequence (ITS) profiling, REP- and ERIC-PCR. ERIC-PCR revealed the presence of seven different genotypes. Amplification of the 16S-23S rDNA spacer region by ITS profiling gave similar results with nine different genotypes. REP-PCR was found to be more discriminatory for typing of Y. enterocolitica than ERIC-PCR and ITS profiling. Fifteen different DNA patterns were obtained by this technique. Based on data obtained by three methods it was found that: (i) Y. enterocolitica strains belonging to the same serotype can represent different genotypes and vice versa; (ii) isolates recovered from humans, pigs and foxes exhibit limited heterogeneity and, independent of the origin, one or two prevailing genotypes were always observed; and (iii) many human Y. enterocolitica isolates shared common genotypes with porcine isolates. |
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