| 冰醋酸法提取猪血红素的研究 |
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| 引用本文： | 胡睿琦,王建敏,王楠,于洋.冰醋酸法提取猪血红素的研究[J].安徽农业科学,2014(16):5211-5213. |
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| 作者姓名： | 胡睿琦 王建敏 王楠 于洋 |
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| 作者单位： | 辽宁医学院; |
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| 基金项目： | 辽宁省“大学生创新创业训练计划”项目(201210160014) |
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| 摘 要： | 目的]合理利用动物血液,优化冰醋酸提取猪血红素的方法.方法]试验以新鲜猪血为原料,采用冰醋酸法提取新鲜猪血中的血红素,考察冰醋酸的添加量、反应温度、反应时间、氯化钠添加量等因素对血红素提取的影响.结果]试验表明,冰醋酸法提取猪血红素的最佳工艺为：冰醋酸添加量为血球4倍,氯化钠添加量为血球的2.4％,反应时间30 min,反应温度为95℃,此条件下提取的血红素含量为40.2％.结论]研究可为猪血资源的合理开发利用提供理论依据.
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| 关 键 词： | 血红素 冰醋酸法 提取 |
Study on the Extraction of Hemoglobin from Porcine Blood by Acetic Acid Method |
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| HU Rui-qiLiaoning Medical University,Jinzhou,Liaoning,WANG Jian-minLiaoning Medical University,Jinzhou,Liaoning,WANG YanLiaoning Medical University,Jinzhou,Liaoning,YU Yang.Study on the Extraction of Hemoglobin from Porcine Blood by Acetic Acid Method[J].Journal of Anhui Agricultural Sciences,2014(16):5211-5213. |
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| Authors: | HU Rui-qiLiaoning Medical University Jinzhou Liaoning WANG Jian-minLiaoning Medical University Jinzhou Liaoning WANG YanLiaoning Medical University Jinzhou Liaoning YU Yang |
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| Institution: | (Liaoning Medical University, Jinzhou, Liaoning 121001) |
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| Abstract: | Objective] To rational use of animal blood,and optimize extraction method of hemoglobin from porcine blood.Method] With fresh porcine blood as raw material,acetic acid method was used to extract hemoglobin from fresh porcine blood,effects of the content of acetic acid,sodium chloride dosage,reaction time and temperature on the extraction of hemoglobin were studied by single factor investigation.Result] The optimum technique is：the optimal extraction for 30 min using acetic acid/porcine red blood cells ratio of4∶1 with sodium chloride of 2.4％ at 95 ℃ gave an actual value of heme yield of 40.2％.Conclusion] The study can provide theoretical basis for rational development and utilization of porcine blood resources. |
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| Keywords: | Hemoglobin Acetic acid method Extraction |
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