| 鸡白细胞介素-18在鸡球虫病疫苗免疫中的作用 |
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| 引用本文： | 陈红英,李新生,崔保安,魏战勇,夏平安,段廷云.鸡白细胞介素-18在鸡球虫病疫苗免疫中的作用[J].江苏农业学报,2009,25(4). |
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| 作者姓名： | 陈红英 李新生 崔保安 魏战勇 夏平安 段廷云 |
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| 作者单位： | 1. 河南农业大学牧医工程学院,河南,郑州,450002
2. 漯河出入境检验检疫局,河南,漯河,462000 |
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| 基金项目： | 国家"十一五"科技支撑计划专项 |
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| 摘 要： | 为了探索鸡白细胞介素-18(IL-18)在鸡球虫病疫苗免疫中的免疫佐剂作用,用鸡IL-18的重组真核表达质粒pIL-l8、柔嫩艾美耳球虫早熟株疫苗及两者联合分别接种3日龄鸡,首免后第1 d、4 d、6 d、9 d、14 d、21 d、26 d、28 d时随机抽取鸡外周血及无菌采取鸡脾脏,应用ELISA和甲基噻唑基四唑(MTT)法分别检测免疫鸡的体液免疫及细胞免疫水平.二免后第14 d用柔嫩艾美耳球虫进行攻击.结果表明,pIL-18与鸡柔嫩艾美耳球虫早熟株疫苗联合免疫具有明显提高虫苗免疫保护的作用,pIL-18单独注射鸡体也具有明显增强鸡体抗球虫感染的作用.表明鸡IL-18能够明显增强鸡球虫病疫苗的免疫原性,显著提高免疫鸡的体液免疫和细胞免疫水平,并且能强有力地抵抗柔嫩艾美耳球虫的攻击.
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| 关 键 词： | 鸡白细胞介素-18 柔嫩艾美耳球虫早熟株疫苗 鸡球虫病 免疫 |
Adjuvant Effect of Chicken Interleukin-18 on the Immune Responses to Coccidiosis Vaccine |
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| CHEN Hong-ying,LI Xin-sheng,CUI Bao-an,WEI Zhan-yong,XIA Ping-an,DUAN Ting-yun.Adjuvant Effect of Chicken Interleukin-18 on the Immune Responses to Coccidiosis Vaccine[J].Jiangsu Journal of Agricultural Sciences,2009,25(4). |
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| Authors: | CHEN Hong-ying LI Xin-sheng CUI Bao-an WEI Zhan-yong XIA Ping-an DUAN Ting-yun |
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| Institution: | 1.College of Animal Husbandry and Veterinary;Henan Agricultural University;Zhengzhou 450002;China;2.Luohe Entry-exit Inspection and Quarantine Bureau;Luohe 462000;China |
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| Abstract: | 3-day-old SPF chickens were inoculated intramuscularly with recombinant plasmid pIL-18,pIL-18 and coccidiosis vaccine or coccidiosis vaccine.Chicken spleen lymphocytes were detected by methyl thiazolyl tetrazolium(MTT)and the sera were collected to examine antibodies of chickens by ELISA on 1 d,4 d,6 d,9 d,14 d,21 d,26 d and 28 d after first immunization.The chickens were challenged on day 14 post the booster.The results showed that chicken IL-18 was able to improve obviously the immunogenicity of coccidios... |
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| Keywords: | chicken interleukin-18 coccidiosis vaccine chicken coccidiosis immunization |
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