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| 马动脉炎病毒大囊膜糖蛋白真核表达载体的构建与瞬时表达 | |
| 引用本文： | 杜建,王志亮,金宁一,张念祖.马动脉炎病毒大囊膜糖蛋白真核表达载体的构建与瞬时表达[J].中国预防兽医学报,2006,28(2):133-135. |
| 作者姓名： | 杜建 王志亮 金宁一 张念祖 |
| 作者单位： | 1. 农业部,动物检疫所,国家外来动物疫病诊断中心,山东,青岛,266032;昆明市畜牧兽医站,云南,昆明,650223 2. 农业部,动物检疫所,国家外来动物疫病诊断中心,山东,青岛,266032 3. 解放军军需大学,病毒基因工程重点实验室,吉林,长春,130062 4. 农业部,热带亚热带动物病毒学重点实验室,云南昆明,650224 |
| 摘 要： | 将马动脉炎病毒大囊膜糖蛋白基因GL插入真核表达载体pVAX1中构建真核表达载体pVAX1-GL，酶切和测序结果表明构建是正确的，用脂质体转染试剂将其转染BHK-21细胞并通过问接免疫荧光试验检测其在体外的表达情况，结果在转染的细胞表面观察到绿色荧光，证明基因得到了表达，而对照则无绿色荧光，本研究为马动脉炎基因疫苗的研究奠定了基础。 |
| 关 键 词： | 马动脉炎病毒 大囊膜糖蛋白 真核表达载体 |
| 文章编号： | 1008-0589（2006）02-0133-03 |
| 收稿时间： | 2004-11-02 |
| 修稿时间： | 2004年11月2日 |
Construction and transient expression of equine arteritis virus major envelope glycoprotein of eukaryotic expression vector |
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| DU Jian,WANG Zhi-liang,JIN Nin-yi,ZHANG Nian-zu.Construction and transient expression of equine arteritis virus major envelope glycoprotein of eukaryotic expression vector[J].Chinese Journal of Preventive Veterinary Medicine,2006,28(2):133-135. | |
| Authors: | DU Jian WANG Zhi-liang JIN Nin-yi ZHANG Nian-zu |
| Abstract: | To construct the eukaryotic expression vector of equine arteritis virus major envelope protein gene and observe its expression in vitro, the recombinant expression vector pVAX1-GL was constructed by inserting the GL gene into the eukaryotic expression vector pVAX1. The pVAX1-GL was transfected into BHK-21 cells by lipofectamine and the expressed product was detected by indirect immunofluorescence. Restriction enzymes digestion analysis and sequencing results revealed that the recombinant expression vector pVAX1-GL has been constructed successfully. The indirect immunofluorescence result showed green fluorescence on the membrane of transfected cells. The constructed eukaryotic expression vector of EAV-GL can be expressed in vitro, which lay the foundation for the further study of EAV DNA vaccine. |
| Keywords: | equine arteritis virus major envelope glycoprotein eukaryotic expression vector |
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