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| 秋子梨品种‘龙香’S基因型的鉴定及梨S_(42)-RNase新基因的序列分析 | |
| 引用本文： | 梁文杰,谭晓风,王立新,夏丽芝,曹玉芬,张琳.秋子梨品种‘龙香’S基因型的鉴定及梨S_(42)-RNase新基因的序列分析[J].北方园艺,2009(12). |
| 作者姓名： | 梁文杰 谭晓风 王立新 夏丽芝 曹玉芬 张琳 |
| 作者单位： | 1. 中南林业科技大学经,济林育种与栽培国家林业局重点实验室,湖南,长沙,410004;温州科技职业学院,浙江,温州,325006 2. 中南林业科技大学经,济林育种与栽培国家林业局重点实验室,湖南,长沙,410004 3. 温州科技职业学院,浙江,温州,325006 4. 中国农业科学院,果树研究所,辽宁,兴城,125100 |
| 基金项目： | 国家林业局重点科学研究计划新技术开发与储备专项资助项目，湖南省科技计划资助项目，国家林业局重点(梨自交不亲和技术的开发与应用)资助项目 |
| 摘 要： | 利用特异引物对秋子梨品种‘龙香'的基因组DNA进行PCR扩增.通过对扩增片段的回收、克隆和测序,获得2条大小分别为469 bp和653 bp的DNA序列,将其推导氨基酸序列与GenBank中已登录的梨S基因的序列进行比对.结果表明:469 bp序列与已登录梨的S16-RNase基因完全一致,确定‘龙香'的1个等位基因为梨S16基因;653 bp序列与梨S基因的相似性在75%～90%之间,且在高变区存在6个以上氨基酸的差异,近一步分析确定为1条新的梨S基因,命名为S42-RNase基因,该基因在GenBank的登录号为:EF088497.确定秋子梨品种‘龙香'的S基因型为S16S42. |
| 关 键 词： | 秋子梨 '龙香' 自交不亲和性 S因型 鉴定 |
Identification of S-genotypes and Sequence Analyses of S42-R Nase from 'LongXiang' (Pyrus ussuriensis ) |
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| LIANG Wen-jie,TAN Xiao-feng,WANG Li-xin,XIA Li-zhi,CAO Yu-fen,ZHANG Lin.Identification of S-genotypes and Sequence Analyses of S42-R Nase from 'LongXiang' (Pyrus ussuriensis )[J].Northern Horticulture,2009(12). | |
| Authors: | LIANG Wen-jie TAN Xiao-feng WANG Li-xin XIA Li-zhi CAO Yu-fen ZHANG Lin |
| Institution: | LIANG Wen-jie1,2,TAN Xiao-feng2,WANG Li-xin2,XIA Li-zhi2,CAO Yu-fen3,ZHANG Lin1(1.The Key Lab of Non-wood Forest Product of State Forestry Administration,Central South University of Forestry , Technology,Changsha,Hunan 410004,China 2.Wenzhou Vocational College of Science , Technology,Wenzhou,Zhejiang 325006,China 3.Fruit Tree Research Institute of Chinese Academy of Agricultural Sciences,Xingcheng,Liaoning 121600,China) |
| Abstract: | Using two pairs of primers specific to pear S-PCR amplification from genomic DNA of Chinese pear cultivar Longxiang' was carried out.Then the PCR products were extracted,cloned and sequenced.Consequently,469 bp and 653 bp DNA sequences of S-RNase were determined.Comparison of the two S-RNase alleles and those deposited under GenBank revealed that the 469 bp S-allele was identical to S16-allele,and that the 653 bp S-allele represents a new allele and was 75%~90% homologous to other pear S-alleles.The new S-a... |
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