| 不同禽流感病毒含量的新-流二联灭活疫苗免疫SPF鸡后抗体水平的监测 |
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| 引用本文： | 江兴华,赵华娥,林晓荣,包松英,崔龙萍,王全溪.不同禽流感病毒含量的新-流二联灭活疫苗免疫SPF鸡后抗体水平的监测[J].福建畜牧兽医,2016(4):22-24. |
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| 作者姓名： | 江兴华 赵华娥 林晓荣 包松英 崔龙萍 王全溪 |
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| 作者单位： | 1. 福州大北农生物技术有限公司 福州 350014;2. 福建农林大学动物科学学院 福州 350002 |
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| 摘 要： | 将未浓缩的新城疫抗原分别与未浓缩的、浓缩3倍、浓缩6倍的禽流感抗原混合，并制备成三组鸡新城疫、禽流感（H9N2 HP株）二联灭活疫苗（简称新-流二联灭活疫苗），分别免疫21日龄SPF鸡，每羽0.3 mL，同时设置未免疫的空白对照组，免疫组与对照组均在免疫前及免疫后7、14、21、28、35 d进行采血，检测新城疫和禽流感抗体。结果发现，各免疫组在免后不同日龄的新城疫抗体基本一致，禽流感病毒抗原浓缩倍数越高（即禽流感病毒含量越高）的新-流二联灭活疫苗，免后14、21 d的抗体也越高；从免后21 d开始，各免疫组的禽流感抗体水平差异逐渐减小，免疫后禽流感抗体水平的高低可以反映该疫苗的免疫效果。试验结果表明，该疫苗可以通过浓缩提高抗原病毒含量的方法来提高免后早期抗体水平，取得良好的早期免疫效果。
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| 关 键 词： | 新城疫 禽流感 不同病毒含量 二联灭活疫苗 SPF鸡 抗体水平监测 |
Detection of antibodies against bivalent inactivated vaccines containing newcastle disease virus and unequal contents of avian influenza virus in SPF chickens |
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| Abstract: | 21-day-old specific pathogen free (SPF) chickens were inoculated with three kinds of bivalent newcastle disease and avian influenza(H9N2 HP strain) inactivated vaccines, which contained original newcastle disease virus(NDV) and primal, three folds of con-centration, six folds of concentration of avian influenza virus(AIV), respectively. Every chicken in immune group was inoculated with a dose of 0.3 mL, and simultaneously, chickens were mock inoculated as control group.In order to detection antibodies of NDV and AIV blood samples were collected from both immune group and control group before immunization and at 0, 7, 14, 21, 28 and 35 day post-immunization(dpi), respectively.Results showed that, the antibody levels against NDV in each immune groups were basically consistent at different timepoints of post-immunization, the antibody levels against AIV increased depending on the virus quantity at 14 and 21 dpi, and then decreased gradually till to non-difference.Overall, antibody levels against AIV could represent the immune effect of this bivalent inactivated vaccines after immunization, and the improvement of antibody levels and immune effect in early immunization might be obtained by means of increasing the amount of antigens. |
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| Keywords: | Newcastle disease Avian influenza Unequal contents of virus Bivalent inactivated vaccines SPF chickens Antibody levels detection |
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