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差异光周期下野牛草相连分株内源激素的日节律
引用本文:李伟,钱永强,韩蕾,孙振元.差异光周期下野牛草相连分株内源激素的日节律[J].草业科学,2017,34(9).
作者姓名:李伟  钱永强  韩蕾  孙振元
作者单位:中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京100091;青岛农业大学园林与林学院,山东青岛266109;中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京100091
基金项目:国家自然科学基金——差异光周期作用下野牛草相连克隆分株间生物节律同步化调控机理
摘    要:为了分析野牛草(Buchloe dactyloides)相连克隆分株在差异光周期下内源激素的变化,利用酶联免疫吸附测定法(ELISA)对野牛草相连克隆分株在相反光照条件下的植物内源激素生长素(IAA)、赤霉素(GA)、脱落酸(ABA)、玉米素核苷(ZR)含量进行了测定,探讨植物内源激素在生物节律调控过程中的作用机理。结果表明,野牛草内源激素IAA、GA、ABA及ZR含量在一天内呈现节律性变化规律,同时,在差异光周期下,野牛草相连克隆分株叶片的内源激素水平趋向于同步化,并且倾向于内源生物节律较强的分株。其中,差异光周期处理1周后,在全光照条件下,野牛草克隆分株在断开状态时,叶片中IAA、GA、ABA及ZR含量在24h内基本呈现相反的变化趋势,而野牛草克隆分株叶片中IAA、GA、ABA及ZR含量在24h内的变化趋于一致。

关 键 词:野牛草  相连分株  差异光周期  内源激素  节律同步化

Circadian rhythm variation of endogenous hormones in Buchloe dactyloides connected ramets under differential photoperiod
Li Wei,Qian Yong-qiang,Han Lei,Sun Zhen-yuan.Circadian rhythm variation of endogenous hormones in Buchloe dactyloides connected ramets under differential photoperiod[J].Pratacultural Science,2017,34(9).
Authors:Li Wei  Qian Yong-qiang  Han Lei  Sun Zhen-yuan
Abstract:In order to understand the endogenous hormonal differences in Buchloe dactyloides connected ramets under different photoperiods,the levels of four endogenous hormones indole-3-acetic acid (IAA),gibberellin (GA),abscisic acid (ABA) and zeatin (ZR)] in buffalo grass leaves were determined using ELISA.The results indicated that IAA,GA,ABA and ZR levels of buffalo grass reveal rhythmic variations on a daily basis,while the endogenous hormone levels of buffalo grass ramets tend to be synchronized even under different photoperiods.After one week of different photoperiod treatments,under continuous light conditions,IAA,GA,ABA and ZR content in disconnected ramets of buffalo grass leaves basically showed the opposite trend within 24 h,however,IAA,GA,ABA and ZR content in connected ramets of buffalo grass leaves were synchronization trend within 24 h.
Keywords:buffalo grass  connected ramets  phytohormone  photoperiod  circadian rhythm synchronization
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