| 玉米秸秆和白菜尾菜混贮料的乳酸菌多样性及耐高温优良菌株筛选 |
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| 引用本文： | 李志忠,窦俊伟,任海伟,李金平,孙文斌,黄娟娟,李梦玉.玉米秸秆和白菜尾菜混贮料的乳酸菌多样性及耐高温优良菌株筛选[J].草业科学,2017,34(6). |
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| 作者姓名： | 李志忠 窦俊伟 任海伟 李金平 孙文斌 黄娟娟 李梦玉 |
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| 作者单位： | 兰州理工大学生命科学与工程学院,甘肃 兰州,730050;兰州理工大学生命科学与工程学院,甘肃 兰州 730050;兰州理工大学西部能源与环境研究中心,甘肃 兰州 730050;甘肃省生物质能与太阳能互补供能系统重点实验室,甘肃 兰州 730050;兰州理工大学西部能源与环境研究中心,甘肃 兰州 730050;甘肃省生物质能与太阳能互补供能系统重点实验室,甘肃 兰州 730050 |
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| 基金项目： | 国家自然科学基金，国家高技术研究发展计划(863)，甘肃省自然科学基金项目，兰州市科技计划项目 |
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| 摘 要： | 通过形态特征、生理生化特性及16S rRNA序列分析方法对玉米(Zea mays)秸秆和白菜(Brassica pekinensis)尾菜混贮料中的乳酸菌多样性进行分析,并以温度和p H为限制因素筛选优良乳酸菌菌株。结果表明,分离得到的12株乳酸菌分属于乳杆菌属(Lactobacillus)和片球菌属(Pediococcus)。其中,1株(LB-1)为植物乳杆菌(Lactobacillus plantarum),6株(LB-2、LB-4、LB-7、LB-8、LB-9和LB-11)为戊糖片球菌(Pediococcus pentosaceus),3株(LB-5、LB-6和LB-12)为短乳杆菌(Lactobacillus brevis),2株(LB-3和LB-10)为类干酪乳杆菌(Lactobacillus paracasei)。菌株LB-3和LB-8表现出优良的耐高温、耐酸碱特性,且具有较强的产乳酸能力,二者可作为青贮饲料的乳酸菌添加剂。
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| 关 键 词： | 玉米秸 尾菜 混合青贮 乳酸菌 多样性 生长特性 |
Lactic acid bacteria diversity analysis and screening of superior thermotolerant strains from corn stover and cabbage waste mixed-silages |
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| Abstract: | Morphological observation, physiological and biochemical tests, and the 16S rRNA sequence analysis methods were used to analyse lactic acid bacteria (LAB) diversity in mixed silage of corn stover and cabbage, and the thermotolerant strains were screened through with temperature and pH as the restrictive indicators.The results showed that 12 LAB strains were isolated from mixed silage, which were classified into Lactobacillus and Pediococcus, respectively.Specifically, one strain (LB-1) was Lactobacillus plantarum, six strains (LB-2, LB-4, LB-7, LB-8, LB-9 and LB-11) were Pediococcus pentosaceus, three strains (LB-5, LB-6 and LB-12) were Lactobacillus brevis, and two strains (LB-3 and LB-10) were Lactobacillus paracasei.The characteristics of LB-3 and LB-8 included high-temperature resistance, acid-resistance and higher acid-production ability.Therefore, these two lactic acid bacteria can be used as inoculants for silage forage. |
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| Keywords: | corn stover vegetable waste mixed silage lactic acid bacteria diversity growth characteristics |
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