| 白颖苔草对不同浓度NaCl胁迫的响应及其耐盐阈值 |
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| 引用本文： | 张昆,李明娜,曹世豪,孙彦.白颖苔草对不同浓度NaCl胁迫的响应及其耐盐阈值[J].草业科学,2017,34(3). |
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| 作者姓名： | 张昆 李明娜 曹世豪 孙彦 |
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| 作者单位： | 中国农业大学动物科技学院草业科学系草业科学北京重点实验室,北京,100093;中国农业大学动物科技学院草业科学系草业科学北京重点实验室,北京,100093;中国农业大学动物科技学院草业科学系草业科学北京重点实验室,北京,100093;中国农业大学动物科技学院草业科学系草业科学北京重点实验室,北京,100093 |
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| 基金项目： | 国家自然科学基金项目“盐胁迫下白颖苔草蛋白组学的研究” |
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| 摘 要： | 以白颖苔草(Carex rigescens)为研究材料,采用盆栽试验研究了不同浓度NaCl(0,100,200,300,400,500mmol·L~(-1))处理下白颖苔草地上部生长和生理等指标的变化趋势,分析了不同浓度盐处理对白颖苔草生长的影响,并计算确定了其耐盐性的阈值。结果表明,盐处理对白颖苔草的生长产生了抑制作用。随NaCl处理浓度增加,白颖苔草的株高、叶长和叶宽均呈下降趋势,同时枯叶率显著升高(P0.05)。白颖苔草叶片相对含水量随NaCl浓度的增加呈下降趋势,而叶片的质膜透性则呈升高趋势。但两指标在低浓度盐(NaCl为100和200mmol·L~(-1))处理下较无胁迫对照无显著差异(P0.05),当NaCl浓度超过300mmol·L~(-1)时,两指标发生显著变化(P0.05)。超氧化物歧化酶(SOD)和过氧化物酶(POD)活性随NaCl浓度的增加呈先升高后降低的趋势,在300mmol·L~(-1)处理下均达到最大值,说明在NaCl浓度300mmol·L~(-1)左右可能存在白颖苔草耐盐的临界浓度。不同浓度NaCl处理对白颖苔草叶片叶绿素a,叶绿素b和叶绿素a+b均产生了不同程度的影响。随盐浓度的增加,叶绿素a和叶绿素a+b含量呈先升高后降低趋把势,叶绿素b含量呈降低趋势,而叶绿素a/b值无显著变化(P0.05)。以白颖苔草生物量下降50%时的盐浓度来评价其耐盐性,建立回归方程确定其耐盐阈值为263mmol·L~(-1)。上述结果表明,白颖苔草对盐胁迫有较高的耐受性,在一定范围的盐胁迫环境中能正常生长,该结果可为后续白颖苔草耐盐机制的研究提供理论依据。
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| 关 键 词： | 白颖苔草 盐胁迫 耐盐性 生长特性 抗氧化酶 叶绿素含量 阈值 |
Response of Carex rigescens to different NaCl concentrations and its salinity threshold calculation |
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| Zhang Kun,Li Ming-na,Cao Shi-hao,Sun Yan.Response of Carex rigescens to different NaCl concentrations and its salinity threshold calculation[J].Pratacultural Science,2017,34(3). |
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| Authors: | Zhang Kun Li Ming-na Cao Shi-hao Sun Yan |
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| Abstract: | The effect of different NaCl concentration (0,100,200,300,400,500 mmol · L-1) treatments on the growth and physiological parameters of Carex rigescens were tested to determine its growth and salinity threshold under salt stress.The results showed that NaCl treatment inhibited the growth of C.rigescens.With increasing salt concentrations,plant height,leaf length,and leaf width of C.rigescens all decreased and the rate of withered leaves increased significantly.Leaf relative water content decreased and leaf membrane permeability increased under the same treatment condition and these two parameters had no significant change under the lower NaCl treatment (100 and 200 mmol · L-1).However,the change was significant when the NaCl concentration exceeded 300 mmol · L-1.The activity of leaf SOD (superoxide dismutase) and POD (peroxidase)increased at first and then decreased and the maximum activity occurred in the 300 mmol · L-1 treatment.This implied that 300 mmol · L-1 could be the critical NaCl concentration for salt tolerance of C.rigescens.Different NaCl concentration treatments had different effects on the chlorophyll content.With the increase in NaCl concentration,chlorophyll a and chlorophyll a+b content increased at first and decreased subsequently.Chlorophyll b tended to decrease and chlorophyll a/b showed no significant change.When C.rigescens biomass decreased to 50％,we considered the NaCl concentration as the threshold to evaluate the salt tolerance of C.duriuscula subsp.rigescens.The calculated salinity threshold value of C.rigescens in our study was 263 mmol · L-1.In conclusion,C.rigescens had a high salt tolerance and it could grow normally in a saline environment.The results of this study provide an important theoretical basis for further research on the salt-tolerance mechanism of C.rigescens. |
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| Keywords: | Carex rigescens salt stress salt tolerance growth characteristics antioxidase chlorophyll content threshold |
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