| 黄瓜花叶病毒外壳蛋白质进入叶绿体与症状发生的关系 |
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| 引用本文： | Randl.,J 朱水芳.黄瓜花叶病毒外壳蛋白质进入叶绿体与症状发生的关系[J].植物病理学报,1992,22(3):229-233. |
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| 作者姓名： | Randl. J 朱水芳 |
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| 作者单位： | 1. 农业部植物检疫实验所, 100026;2. 中国科学院微生物研究所, 100080;3. 澳大利亚Adel ai de大学, 5064 |
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| 摘 要： | 由感染黄瓜花叶病毒(CMV)、健康和转外壳蛋白(CP)基因的烟叶以原生质体法制备纯化的叶绿体。经SDS-PAGE电泳,银染色,比较其蛋白质图谱。用CMV外壳蛋白游离亚基制备的抗血清进行Western blotting。结果发现:1.CP存在于CMV侵染的烟叶绿体中。2.叶绿体中CP的浓度和花叶症状严重程度呈正相关;3.表达CP的转基因烟草叶绿体中未测出CP存在。根据以上结果,认为CMV侵染的烟花叶症状产生与CP进入叶绿体有直接相关性。
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| 关 键 词： | 黄瓜 花叶病 病毒 蛋白 叶绿体 |
THE PRESENCE OF CUCUMBER MOSAIC VIRUS COAT PROTEIN IN CHLOROPLASTS IS RELATED TO THE EXPRESSION OF MOSAIC SYMPTOM ON TOBACCO |
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| Zhu Shuifang,Ye Yin,Zhao Feng,Tian Bo,John Randle,R. I. B. Francki.THE PRESENCE OF CUCUMBER MOSAIC VIRUS COAT PROTEIN IN CHLOROPLASTS IS RELATED TO THE EXPRESSION OF MOSAIC SYMPTOM ON TOBACCO[J].Acta Phytopathologica Sinica,1992,22(3):229-233. |
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| Authors: | Zhu Shuifang Ye Yin Zhao Feng Tian Bo John Randle R I B Francki |
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| Institution: | 1. Plant Quaantine Institute, Ministry of Agriculture Beijing, 100026;2. Institute of Microbiology, Academia Sinica, Beijing, 100080;3. The University of Adelaide, Adelaide, Australia 5064 |
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| Abstract: | The pure intaot chloroplasts were isolated from protoplasts released from tobacco leaves infected by Cucumber Mosaic Virus(CMV),or from the transgenic tobacco leaves expressing CMV coat protein(CP).The CP was detected by Western Blotting using the antiserum agaist CMV CP subunit.We concluded that:1,CP was found inside chloroplasts of CMV infectedtobacco;2.The concentration of CP inside chloroplasts was positively related to the severity of the symptom;3.CP was not associated with the chloroplasts from the transgenic tobacco.It was suggested that the presence of CP inside chloroplasts is related to the Mosaic symptom on tobacco. |
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