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海洋低温碱性蛋白酶的刺激性研究
引用本文:房丽华,张健,仲伟珍,闫凤霞,姚如永,于国英,张云波,刘晓萍,王春波.海洋低温碱性蛋白酶的刺激性研究[J].海洋水产研究,2000,21(4):94-101.
作者姓名:房丽华  张健  仲伟珍  闫凤霞  姚如永  于国英  张云波  刘晓萍  王春波
作者单位:1. 青岛大学医学院,266021
2. 青岛大学医学院附属医院,266003
3. 中国水产科学研究院黄海水产研究所 海洋酶与酶工程开放实验室,青岛 266071
基金项目:国家“海洋863”项目(863-819-06-01)资助
摘    要:探究海洋低温碱性蛋白酶的刺激性,并为其应用提供实验依据。作者应用新西兰大耳白兔,以40%、20%和5‰浓度的海洋低温碱性蛋白酶进行皮肤急性刺激试验,5‰海洋低温碱性蛋白酶的皮肤多次刺激性试验,1‰的海洋低温碱性蛋白酶的兔角膜刺激性试验,评价其安全性。结果表明,5‰和20%海洋碱性蛋白酶单次应用实验皮肤未出现红斑和水肿,对皮肤无刺激性。40%海洋低温碱性蛋白酶液对皮肤有中度刺激性。皮肤病理学显示5‰的海洋低温碱性蛋白酶液未见皮肤的慢性刺激反应,皮肤结构完整、层次清晰。1‰的海洋低温碱性蛋白酶液对兔眼亦无刺激性。

关 键 词:海洋低温碱性蛋白酶  白兔  刺激性反应  洗涤剂

Irritation study on the marine low-temperature alkaline proteinase
FANG Li-hua,ZHANG Jian,ZHONG Wei-Zhen,YAN Feng-xia,YAO Ru-yong,YU Guo-ying,ZHANG Yun-bo,LIU Xiao-ping,WANG Chun-bo.Irritation study on the marine low-temperature alkaline proteinase[J].Marine Fisheries Research,2000,21(4):94-101.
Authors:FANG Li-hua  ZHANG Jian  ZHONG Wei-Zhen  YAN Feng-xia  YAO Ru-yong  YU Guo-ying  ZHANG Yun-bo  LIU Xiao-ping  WANG Chun-bo
Abstract:To further study the irritation of marine low-temperature alkaline proteinase and provide experimental data for its utilization. The safety of marine low-temperature alkaline proteinase was evaluated by acute and chronic dermal irritation and acute eye irritation on rabbits. The results show that the skin experiment with marine low-temperature alkaline proteinase (5‰,20%) dont cause swollen and redness of the skin. It has no irritation effect on the skin. The marine low-temperature alkaline proteinase (40%) has middle irritation on the skin. Pathologic study shows that no chronic irritation effect of marine low-temperature alkaline proteinase(5‰)on the skin has been found. The 1‰ solution for marine low-temperature alkaline proteinase has no accute irritation effect on the eye
Keywords:Marine  low  temperature alkaline proteinase    New Zealand rabbit    Irritation
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