Amenability of castor to an <Emphasis Type="Italic">Agrobacterium</Emphasis>-mediated <Emphasis Type="Italic">in planta</Emphasis> transformation strategy using a <Emphasis Type="Italic">cry1AcF</Emphasis> gene for insect tolerance |
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| Authors: | Arthikala Manoj Kumar Rohini Sreevathsa Kalpana Nanja Reddy Prasa Trichy Ganesh Makarla Udayakumar |
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| Institution: | (1) Department of Crop Physiology, University of Agricultural Science, GKVK, Bangalore, 560065, India;(2) Present address: Department of Entomology, University of Agricultural Sciences, GKVK Campus, Bangalore, 560065, India; |
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| Abstract: | Agrobacterium tumefaciens mediated in planta transformation protocol was developed for castor, Ricinus communis. Two-day-old seedlings were infected with Agrobacterium strain EHA105/pBinBt8 harboring cry1AcF and established in the greenhouse. Screening the T1 generation seedlings on 300 mg L−1 kanamycin identified the putative transformants. Molecular and expression analysis confirmed the transgenic nature and identified
high-expressing plants. Western blot analysis confirmed the co-integration of the nptII gene in the selected transgenic plants. Bioassay against Spodoptera litura corroborated with high expression and identified five promising effective lines. Analysis of the T2 generation plants proved the stability of the transgene indicating the feasibility of the method. |
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