Construction of a Fosmid genomic library of Streptomyces roseoflavus Men-myco-93-63 |
| |
| Authors: | Weiming Sun Wei Guo Daqun Liu Tinghui Liu Lina Feng Lianna Liu Yaning Li Yakun Zhang |
| |
| Institution: | (1) Department of Pathology, Stanford University School of Medicine, Stanford CA 94305, and Hopkins Marine Station, Pacific Grove, CA 93950, USA |
| |
| Abstract: | To clone the antibiotic biosynthesis gene cluster of Streptomyces roseoflavus Men-myco-93-63, we constructed a Fosmid genomic library. The genomic DNA of the strain Men-myco-93-63 was isolated by the
modified CTAB procedure, and the size of most genomic DNA fragments was larger than 150 kb. Then, a Fosmid genomic library
containing more than 6000 clones was constructed. The average size of the inserted DNA in recombinant plasmids was 38.1 kb,
and the probability of harboring any gene in the genome of the strain Men-myco-93-63 was 99.99%. The library coverage was
at least a 10-fold genome equivalent. Therefore, the constructed Fosmid library meets the requirements as a standard genomic
library |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
