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8种大花蕙兰耐热性指标筛选及其评价
引用本文:周桂英,王四清,许建新,陈卿然,马川.8种大花蕙兰耐热性指标筛选及其评价[J].安徽农业科学,2016,44(16).
作者姓名:周桂英  王四清  许建新  陈卿然  马川
作者单位:深圳市铁汉生态环境股份有限公司,广东深圳,518040;北京林业大学,北京,100083
基金项目:广东省省级科技计划项目(2015B090904008),广东省软科学研究计划项目(2014B090903015),广东省生态环境建设与保护(铁汉)工程技术研究中心项目[粤科函政字(2013)1589号]。
摘    要:目的]筛选并评价大花蕙兰品种的耐热性指标。方法]以8个大花蕙兰品种的1年生小苗为试材,观测在40/30℃(日温/夜温)高温条件下其热害指数、叶绿素含量、初始荧光值(F0)和PSⅡ系统最大光化学效率(Fv/Fm)的变化,并结合相关性分析评价8种大花蕙兰的耐热性强弱及筛选出有效耐热评价指标。结果]1高温胁迫使Fv/Fm、叶绿素a含量、叶绿素b含量下降,且胁迫时间越长,下降越多;2耐热性强的金玉满堂、马可受高温胁迫Fv/Fm下降较小,且在恢复适温后能迅速恢复到初始值的90%以上,而耐热性差的福娘和修女Fv/Fm受高温胁迫显著下降,且在恢复适温后无法恢复;38个品种耐热性从强到弱为:金玉满堂、马可、玉蝉、UFO、红霞、黄金岁月、修女、福娘。结论]研究发现,Fv/Fm与植物的热害指数相关系数达0.946,可作为大花蕙兰耐热品种筛选的可靠指标之一。

关 键 词:大花蕙兰  耐热性  筛选  评价

Heat Resistance Indexes Identification and Comprehensive Evaluation of 8 Species of Cymbidium hybridium
Abstract:Objective] The aim was to screen out and evaluate heat resistance indexes of Cymbidium hybridum. Method] With 1 year old seed-lings of 8 C. hybridum as test materials, the change of heat injury index, chlorophyll content, Fv/Fm and F0 under 40/30 ℃ temperature was observed. The data was treated by correlation analysis for their comprehensive evaluation of heat tolerance and effective heat resistance index. Result] The results showed that①chlorophyll a, chlorophyll b, Fv/Fm decreased with the extension of treatment time;②Fv/Fm of Jinyuman-tang and Make decreased in small scale and recovered to 90% of the initial value quickly as the temperature returned, which had the strongest hardiness;Funiang and Xiunv had the worst hardiness;③The heat resistance of 8 varieties of C. hybridium from strong to weak were:Jinyuman-tang, Make, Yuchan,UFO, Hongxia, Huangjinsuiyue, Xiunv, Funiang. Conclusion] The correlation coefficient of Fv/Fm and heat damage in-dex is 0. 946, which can be used as one of the reliable indexes of heat tolerance variety of C. hybridium.
Keywords:Cymbidium hybridum  Heat resistance  Screening  Evaluation
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