| 尾巨桉3个无性系外植体诱导技术研究 |
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| 引用本文： | 石前,梁秀莉,李飞宇,邓冬丽,李丽芳,李俏.尾巨桉3个无性系外植体诱导技术研究[J].安徽农业科学,2016,44(15). |
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| 作者姓名： | 石前 梁秀莉 李飞宇 邓冬丽 李丽芳 李俏 |
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| 作者单位： | 广西壮族自治区国有东门林场,广西扶绥,532108;中种国际种子有限公司,北京,100028 |
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| 摘 要： | 目的]研究尾巨桉3个无性系的外植体诱导相关技术。方法]筛选最佳采集季节、最优取材部位、最佳消毒时间和最优植物生长调节剂(6-BA)浓度,提高尾巨桉无性系外植体的萌芽率。结果]3个无性系最佳诱导季节为春秋两季;外植体诱导取材的最佳部位是半木质化的枝条;DH32-26、DH32-28外植体的最佳消毒时间为7 min、DH32-29外植体的最佳消毒时间为5 min;适宜外植体诱导的植物生长调节剂组合为6-BA 1.0 mg/L+NAA 0.4 mg/L,此时诱导萌芽率高。结论]要提高外植体诱导的成功率,需从采集季节、取材部位、消毒时间以及植物生长调节剂组合方面综合考虑,该研究为尾巨桉苗木快速繁殖提供了理论依据。
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| 关 键 词： | 尾巨桉 无性系 外植体诱导 |
Study on Explant Induction of Different Hybrids of Eucalyptus urophylla ×E.grandis Clones |
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| Abstract: | Objective] The aim was to study explant induction relevant technology of Eucalyptus urophylla ×E.grandis three clones.Meth-od] The optimal collection season, sampling part, disinfection time and concentration of plant growth regulator(6-BA) was selected, so as to im-prove germination rate of explants of Eucalyptus urophylla × E.grandis clones. Result] The spring and autumn were the optimal seasons for the explant induction, semi-lignified branches was the most suitable part of explant sampling , the most appropriate disinfection duration with mer-curic chloride is 7 minutes for DH32-26 and DH32-28, and 5 minutes for DH32-29.6-BA1.0 mg/L and NAA0.4 mg/L as plant growth regulator combination was good for explant induction which with the best germination rate.Conclusion] In order to improve the successful rate of explant induction, a comprehensive consideration should be made in terms of the collection season, sampling part, disinfection time and plant growth reg-ulator combination.The study can provide theoretical basis for rapid propagation of Eucalyptus urophylla × E.grandis seedlings. |
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| Keywords: | Eucalyptus urophylla ×E grandis Clone Explant induction |
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