| 北五味子“红珍珠”组织培养研究 |
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| 引用本文： | 于敦亮,邢亚娟,殷东生,张建瑛,田新华.北五味子“红珍珠”组织培养研究[J].安徽农业科学,2016,44(15). |
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| 作者姓名： | 于敦亮 邢亚娟 殷东生 张建瑛 田新华 |
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| 作者单位： | 牡丹江林区广播大学,黑龙江牡丹江,157010;黑龙江省林业研究所,黑龙江哈尔滨,150080 |
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| 基金项目： | 黑龙江省属科研院所基本科研业务费专项；黑龙江省科技厅创新团队项目(YC2014D005)。 |
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| 摘 要： | 目的]研究适合北五味子"红珍珠"组织培养的方法。方法]以北五味子"红珍珠"为试材,研究不同外植体、激素对其组织培养诱导、分化和生根的影响。结果]以春季嫩枝为外植体,分化培养基为1/2MS+6-BA 2.0 mg/L+NAA 0.01 mg/L+ZT0.1 mg/L,以1/2MS+IAA 1.0 mg/L+IBA 2.0 mg/L生根效果较好,有利于北五味子"红珍珠"根系的生长。结论]筛选出北五味子"红珍珠"适宜的组织培养配方,为其大规模工厂化育苗提供技术支撑。
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| 关 键 词： | 北五味子 组织培养 培养基 |
Tissue Culture Research of Schisandra chinensis ‘Red Pearl’ |
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| Abstract: | Objective] The aim was to obtain the suitable tissue culture method for Schisandra chinensis‘Red pearl’ . Method] The effects of different explants and hormones on the induction, differentiation and rooting of tissue culture were studied. Result] With spring shoots as explants, the differentiation culture medium was 1/2MS +6-BA 2.0 mg/L +NAA 0.01 mg/L +ZT 0.1 mg/L.1/2MS+IAA 1.0 mg/L+IBA 2.0 mg/L had better rooting effect, which was conducive to the growth of root system of Schisandra chinensis‘Red pearl’.Conclu-sion] The suitable tissue culture formula of Schisandra chinensis‘Red pearl’ is selected, which can provide the technical support for large-scale breeding. |
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| Keywords: | Schisandra chinensis Tissue culture Culture medium |
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