| 鱼油乙酯的制备·纯化及降血脂功效的研究 |
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| 引用本文： | 刘妍,李晓璐,郭艳峰,谭贵良.鱼油乙酯的制备·纯化及降血脂功效的研究[J].安徽农业科学,2016,44(19):80-83. |
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| 作者姓名： | 刘妍 李晓璐 郭艳峰 谭贵良 |
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| 作者单位： | 中山火炬职业技术学院生物医药系,广东中山,528436;中山市食品药品检验所,广东中山,528437 |
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| 基金项目： | 国家质检总局科技计划项目(2014QK051)；中山市科技计划项目(2014A2FC247)。 |
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| 摘 要： | 目的]研究鱼油乙酯的制备、纯化及其降血脂功效。方法]以鱼油为原料,采用碱催化制备了鱼油乙酯,优化了条件并对其进行纯化,将产品配成饲料进行动物试验,研究其降血脂功效。结果]试验表明,乙醇-鱼油6∶1(摩尔比),反应温度75℃,反应时间2h,碱催化剂Na OH加入量为油重的0.30%;在此条件下鱼油脂肪酸乙酯得率最高,经分子蒸馏后达95%;硝酸银最佳浓度为75%,提取得到的二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)总浓度96%~99%;高含量EPA和DHA鱼油能够显著降低小鼠血清中的总胆固醇(TC)、甘油三酯(TG)和低密度脂蛋白胆固醇(LDL-C)浓度,提高高密度脂蛋白胆固醇(HDL-C)浓度,可有效改善高脂血症小鼠的血脂水平。结论]研究可为鱼油的深加工开发利用提供参考。
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| 关 键 词： | 鱼油乙酯 制备 纯化 降血脂 |
Preparation and Purification of Fish Oil Fatty Acid Ethyl Esters and Their Effects on Blood Lipid Reduction |
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| Abstract: | Objective] To study the preparation and purification of fish oil fatty acid ethyl esters and their effects on blood lipid reduction. Method] Fish oil fatty acid ethyl esters were made from fish oil by base catalysis and purified , and the conditions for the ethyl esters produc-tion were optimized.Effects of high levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on blood lipid reduction were studied.Result] The optimum conditions for ethyl esters production were shown as follows:the molar ratio of ethanol to fish oil was 6∶1;re-action temperature was 75 ℃; reaction time was 2 h;NaOH concentration was 0.30%.After molecular distillation, the yield of the ethyl es-ters was up to 95%under the optimum conditions.DHA and EPA were extracted by silver nitrate-water method, and total content of DHA and EPA was up to 96%-99% when AgNO3 concentration was 75%.High levels of DHA and EPA significantly decreased the contents of serum total cholesterol (TC), triglyceride (TG) and low density lipoprotein cholesterin (LDL-C)and improved the content of high density lipoprotein cholesterin (HDL-C), which indicating that high levels of DHA and EPA could improve the disturbance of blood lipid on hyperlipidemia mice.Conclusion] The study can provide scientific references for the deep processing, exploitation and utilization of fish oil. |
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| Keywords: | Fish oil fatty acid ethyl esters Preparation Purification Blood lipid reduction |
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