| UPLC－MS／MS法检测不同产地野生天麻中天麻素及对羟基苯甲醇含量 |
| |
| 引用本文： | 秦伟瀚,师萱,花雷,白孝勇,张左香,舒兴勇,郭延垒,阳勇.UPLC－MS／MS法检测不同产地野生天麻中天麻素及对羟基苯甲醇含量[J].安徽农业科学,2016,44(17):143-145. |
| |
| 作者姓名： | 秦伟瀚 师萱 花雷 白孝勇 张左香 舒兴勇 郭延垒 阳勇 |
| |
| 作者单位： | 重庆市中药研究院,重庆,400065;云南省威信县天麻产业发展办公室,云南威信,657900 |
| |
| 摘 要： | 目的]采用超高效液相色谱质谱联用法同时测定6个产地天麻中天麻素和对羟基苯甲醇含量。方法]采用Phenomenex C18柱(50 mm×2.1 mm,3μm),乙腈-5 mmol/L乙酸铵(0.1%甲酸)水溶液作流动相,流速0.2 m L/min;多反应监测正离子模式(MRMPos)。结果]天麻素在0.122 0~1 220.000 0 ng/m L、对羟基苯甲醇在0.502 0~5 020.000 0 ng/m L浓度范围内呈良好的线性关系。所有样品中天麻素和对羟基苯甲醇的含量均超过《中国药典》2015年版中"天麻"含量测定要求,按照2种成分总含量排序从大到小依次为云南昭通、贵州毕节、四川雅安、重庆城口、陕西汉中、甘肃陇南。结论]该方法精密度、重现性和分离效果好,分析快速、准确,适用于对天麻中天麻素和对羟基苯甲醇的定性、定量研究。
|
| 关 键 词： | 天麻 超高效液相色谱-质谱/质谱联用 天麻素 对羟基苯甲醇 含量 |
Determination of Gastrodine and 4-Hydroxybenzyl alcohol in Gastrodia elata Bl.from Different Habitats by UPLC-MS/MS |
| |
| Abstract: | Objective] To develop an UPLC-MS/MS method for simultaneous determination of gastrodine and 4-hydroxybenzyl alcohol in Gastro-dia elata Bl.from six different habitats.Method] The analyses were performed on a Phenomenex C18 column (50 mm ×2.1 mm, 3 μm) with a mobile phase of acetonitrile -5 mmol/L ammonium acetate aqueous (0.1% formic acid) at a flow rate of 0.2 mL/min operating in MRM posi-tive ion mode (MRM-Pos).Result] Gastrodine and 4-hydroxybenzyl alcohol contents showed good linearity in ranges of 0.122 0-1 220.000 0 and 0.502 0-5 020.000 0 ng/mL, respectively.In all the samples, gastrodine and 4-hydroxybenzyl alcohol contents all exceeded the content determination requirements in Chinese Pharmacopoeia (2015 edition).These herbs of Gastrodia elata Bl.could be ordered according to the total content of gastrodine and 4-hydroxybenzyl alcohol:Zhaotong in Yunnan >Bijie in Guizhou >Ya’an in Sichuan >Chengkou in Chongqing >Hanzhong in Shanxi >Longnan in Gansu.Conclusion] This method is quick, precise, rapid and accurate, and is suitable for the qualitative and quantitative research on gastrodine and 4-hydroxybenzyl alcohol in G.elata. |
| |
| Keywords: | Gastrodia elata Bl UPLC-MS/MS Gastrodine 4-Hydroxybenzyl alcohol Content |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
