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| 仿刺参基因组大小的测定 | |
| 引用本文： | 刘 进,张晓军,苏 琳,刘石林,汝少国,杨红生.仿刺参基因组大小的测定[J].水产学报,2012,36(5):686-695. |
| 作者姓名： | 刘 进 张晓军 苏 琳 刘石林 汝少国 杨红生 |
| 作者单位： | 1. 中国海洋大学海洋生命学院,山东青岛266003;中国科学院海洋研究所,海洋生态与环境科学重点实验室,山东青岛266071 2. 中国科学院海洋研究所,海洋生态与环境科学重点实验室,山东青岛266071 3. 中国科学院海洋研究所,海洋生态与环境科学重点实验室,山东青岛266071;中国科学院研究生院,北京100049 4. 中国海洋大学海洋生命学院,山东青岛,266003 |
| 基金项目： | 国家“八六三”高技术研究发展计划(2012AA10A412); 国家自然科学基金项目(41076103,40976089,41176139) |
| 摘 要： | 仿刺参基因组大小测定采用流式细胞术,以鸡红血细胞DNA含量(2.5 pg/2C)为内标,用夏眠、正常和野生3个群体的仿刺参体腔细胞测定了52只刺参的单倍体基因组含量,其C-值为(0.90±0.06)pg,由此得出仿刺参基因组大小为(880.2±58.68)Mb。其中,夏眠、正常和野生群体C-值分别为(0.93±0.05)、(0.90±0.05)和(0.84±0.02)pg。运用独立样本t检验对比分析显示:(1)夏眠与正常的仿刺参基因组大小之间差异不显著,说明仿刺参基因组大小与夏眠这一生理因子不具有相关性;(2)池塘(围堰)养殖环境下与野生环境下的仿刺参基因组大小之间有显著差异,野生环境下的仿刺参基因组明显小于池塘(围堰)养殖环境下的基因组。据此推测,环境因子的改变可能对仿刺参乃至整个海参类群基因组DNA含量产生影响。实验还对C-值测定的发展和方法作了详尽的描述,同时在综合其他种类海参基因组大小研究的基础上,探讨了C-值与进化地位之间的关系。 |
| 关 键 词： | 仿刺参 基因组大小 流式细胞术 |
| 收稿时间： | 9/29/2011 8:47:19 PM |
| 修稿时间： | 2/6/2012 10:47:39 PM |
Genome size determination of sea cucumber (Apostichopus japonicus) |
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| LIU Jin,ZHANG Xiao-jun,SU Lin,LIU Shi-lin,RU Shao-guo and YANG Hong-sheng.Genome size determination of sea cucumber (Apostichopus japonicus)[J].Journal of Fisheries of China,2012,36(5):686-695. | |
| Authors: | LIU Jin ZHANG Xiao-jun SU Lin LIU Shi-lin RU Shao-guo and YANG Hong-sheng |
| Institution: | Ocean University of China,Institute of Oceanology, Chinese Academy of Science,Institute of Oceanology, Chinese Academy of Science,Ocean University of China |
| Abstract: | Sea cucumber (Apostichopus japonicus) is one of the most important economic aquaculture species in China. The study on the genome size(C-value) is highly significant because it plays an important role in instructing the hybridization breeding and improved variety breeding program, as well as in estimating the cost of the whole genome sequencing project. Previous studies mainly focused on these research areas such as ecology, physiology, nutrition etc. However, it is relatively scarce for the accumulation of basic data related to the genome size and characteristics in A. japonipus. Therefore, the measure of genome size for enriching cytogenetics data of this species is meaningful. In this paper, the genome size(C-value) of sea cucumber, A. japonicus was measured through flow cytometry in 52 individuals, which came from aestivation, normal and wild groups, using erythrocyte DNA content of chicken, Gallus domesticus(2.5pg/2C) as internal standard. The result indicates that the C-value of A. japonicus is (0.90± 0.06) pg, equivalent to (880.2±72.26) Mb. And,the C-values of aestivation, normal and wild are (0.93±0.05) pg, (0.90±0.05) pg and (0.84±0.02) pg, respectively. In addition, analyzed by Independent-Samples T Test, it shows that the C-values have no significant difference in aestivation and normal groups, whereas, the C-values of wild group and that reared in captivity group have significant difference. In other words, there is no correlation between genome size and the physiology factor-aestivation, while the stability of environment factors has certain influences on genome size variation. Moreover, the development of C-value measure method was elucidated in detail, in the meanwhile, the relationship between C-value and the evolution in Holothuroidea was discussed in combination with genome size of other types of sea cucumber. |
| Keywords: | Apostichopus japonicus genome size flow cytometry |
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