| 濒危植物夏蜡梅遗传分化研究 |
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| 引用本文： | 谈 探
,,金则新
ˇ,李钧敏
,张文标
.濒危植物夏蜡梅遗传分化研究[J].西北林学院学报,2008,23(3). |
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| 作者姓名： | 谈 探 金则新
ˇ 李钧敏 张文标 |
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| 作者单位： | 1.北京林业大学自然保护区学院， 北京 100083;2.台州学院生态研究所， 浙江 临海
317000 |
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| 摘 要： | 为阐明小尺度范围内濒危物种夏蜡梅种群的遗传分化，保护和恢复夏蜡梅种质资
源，采用
ISSR分子标记技术，对浙江省临安市清凉峰镇的5个夏蜡梅种群的遗传结构进行分析。1
2个引
物对夏蜡梅5个种群100个个体进行扩增，共得到171个位点，其中多态位点98个，总多态
位点百
分率（P）为 57.31%，平均为25.03%。夏蜡梅总Shannon指数 （I）为0.268 9，平均为
0.148 8; 总
Nei指数 （h）为0.174 5，平均为0.102 8。 P、I、h均表明夏蜡梅总体水平的遗传多
样性较高，而种群
水平的遗传多样性较低。AMOVA分子变异分析结果显示，47.01%的变异存在于种群间，5
2.99%
的变异存在于种群内;种群内与种群间的遗传分化均很明显。夏蜡梅种群间基因流较低，
为0.717
3，显示夏蜡梅种群间的基因流动部分受阻。 5个种群的平均遗传距离为0.1051，根据
种群间的遗
传距离进行UPGMA聚类，结果为生境相似的种群聚在一起。夏蜡梅种群间较高水平的遗传
分化
主要是本身的生物学特性以及对微生境适应的结果。
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| 关 键 词： | 夏蜡梅 ISSR 遗传多样性 遗传分化 |
Genetic Differentiation of Calycanthus chinensis an Endangered Plant |
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| TAN Tan
,,JIN Zeˉxin
ˇ,LI Junˉmin
,ZHANG Wenˉbiao
.Genetic Differentiation of Calycanthus chinensis an Endangered Plant[J].Journal of Northwest Forestry University,2008,23(3). |
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| Authors: | TAN Tan JIN Zeˉxin
ˇ LI Junˉmin ZHANG Wenˉbiao |
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| Institution: | 1.College of Natural Conservation， Bejing Forestry University， Bei
jing100083， China;
2.Institute of Ecology， Taizhou University， Linhai， Zhejiang 317000， C
hina |
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| Abstract: | Genetic structures of 5Calycanthus chinensis populations，locate
d Qingliangfeng in Lin'an city
Zhejiang Province， were analyzed by Using Inter Simple Squence Repeat（
ISSR） molecular marker techˉ
nique，to reved genetic differentiation of S. chinensis fine scale，and t
o provided a theoretical basis for proˉ
tecting and restoring its germplasm resources. Using12 primers， 98 pol
ymorphic loci from total 171 loci
were detected in 100 individuals from 5 populations and the overall p
ercentage of polymorphic loci（P）was
57.31%with an average of 25.03%.Total Shannon's information index（I）
and Nei's gene diversity（h）of
S. chinensis was the total value of0.268 9、0.174 5 with an average o
f 0.148 8、0.102 8respectively. The
results of P， I and h indicated that genetic diversity at species l
evel was relatively high， but at relatively
low at population level. Analysis of molecular variation （AMOVA） su
ggested the variation was 47.01% aˉ
mong populations and 52.99% within population. Therefore， genetic diffe
rentiation within population and
among populations were significant. The gene flow among populations
was as low as 0.717 3indicating
that part of gene flow was blocked. The mean genetic distance of 5
S. chinensis populations was
0.105 1 .
Based on genetic distance， the clustered result for 5S. chinensis po
pulations by unweighted pair group
method arithmetic average （UPGMA） suggested the populations in the
similar habitat were clustered toˉ
gether. So genetic differentiation among S. chinensis populations was i
nduced by its biological characterisˉ
=================================.77.=================================
tic and the adaptability for different microhabitat. |
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| Keywords: | Calycanthus chinensis ISSR genetic diversity genetic differentiation |
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