Open-design Recirculating Systems for Zebrafish Culture |
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| Institution: | 1. Aix Marseille Université, CNRS, Centrale Marseille, M2P2 UMR 7340, Equipe Procédés Membranaires (EPM), Europôle de l’Arbois, BP80, Pavillon Laennec, Hall C, 13545 Aix en Provence Cedex, France;2. Plateforme expérimentale Mollusques Marins, Station Ifremer de Bouin, Polder des Champs, 85230 Bouin, France;3. Suez - Aquasource, 20, avenue Didier Daurat, 31029 Toulouse cedex 04, France;1. Department of Biological and Environmental Engineering, Cornell University, Riley-Robb Hall, Ithaca, NY 14853, USA;2. The Conservation Fund, Shepherdstown, WV 25443, USA;1. Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo, 1138656, Japan;2. Institute of Industrial Science, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba, 2778574, Japan |
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| Abstract: | The zebrafish is an established vertebrate-animal model in biomedical research. Currently, their mass culture is mainly done using systems provided by commercial suppliers. Commercial systems are compact, recirculating, and use auto-cleaning tanks. These features minimize space use, labour cost, and water wastage; thus, facilitating maintenance of a large number of zebrafish using minimal resources. However, the often considered costs associated with these systems often impose a barrier to current and prospective researchers, especially those with limited funds or working in labs with no access to institutional centralized zebrafish culture facilities. In contrast to commercial systems, custom-made zebrafish maintenance systems are also described in the literature. To distinguish custom-made system from commercial systems, we termed them as “open-design” systems. Open-design systems are cost-effective, modular, and frequently being improved by zebrafish researchers. However, for further development and to present them as a viable option for zebrafish researchers around the world, a review of their current status and technical understanding is required. Here, we compile the disparate data on the purpose and technological development of the open-design systems. We believe this review will be a valuable resource for all zebrafish users and help streamline the open-design technology for zebrafish culture. |
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| Keywords: | Custom-made Fish facility Open-design Recirculating Self-cleaning Zebrafish maintenance |
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