| 磺胺间二甲氧嘧啶(SDM)单克隆抗体的制备及初步鉴定 |
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| 引用本文： | 何方洋,罗晓琴,李静,刘琳,冯才伟,万宇平.磺胺间二甲氧嘧啶(SDM)单克隆抗体的制备及初步鉴定[J].现代农业科技,2010(3):322-323. |
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| 作者姓名： | 何方洋 罗晓琴 李静 刘琳 冯才伟 万宇平 |
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| 作者单位： | 北京望尔生物技术有限公司,北京,102206 |
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| 摘 要： | 用磺胺间二甲氧嘧啶人工免疫原(BSA-SDM)免疫Balb/c小鼠,利用细胞融合技术筛选抗磺胺间二甲氧嘧啶单克隆抗体(SDMmAb)杂交瘤细胞,体内诱生腹水法制备SDMmAb,并鉴定其免疫学特性;鉴定结果表明,筛选出3株杂交瘤细胞,单克隆抗体的蛋白亚型为IgG1;分子量为178.1KDa,染色体数目为76～87条。与其他7种磺胺药(SD、SMM、SMZ、SQ、ST、PST、SMT、SPD)有很高的交叉反应性,可用于推广磺胺类药物多残留快速检测试剂盒的研制应用。
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| 关 键 词： | 磺胺间二甲氧嘧啶 ELISA 细胞融合 单克隆抗体 |
Preparation and Identification of Monoclonal Antibodies Against Sulfadlmethdme |
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| Authors: | HE Fang-yang LUO Xiao-qin LI Jing LIU Lin FENG Cai-wei WAN Yu-ping |
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| Institution: | Beijing Wanger Biotechnology Company;Beijing 102206 |
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| Abstract: | Balb/c mice were immunized with bovine serum albumin and sulfadlmethdme(BSA-SDM),and hybridoma lines that secrete monoclonal antibody against sulfadlmethdme(SDMmAb)were filtered with cell fusion,and the immunological traits of SDMmAb were characterized.The results showed that three hybridoma lines were filtered.The antibody of subclasses IgG1 was isolated.The molecular weight was 178.1Kda,the number of chromosome of the hybridoma cell line was from 76 to 87.Cross-reactivity between the antibody and the othe... |
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| Keywords: | sulfadlmethdme ELISA cell fusion monoclonal antibody |
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