| 玉米自交系X178、掖478授粉前后miRNA表达差异分析 |
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| 引用本文： | 王琳琳,赵永平,张从省,杨海龙,王宏伟,谢传晓.玉米自交系X178、掖478授粉前后miRNA表达差异分析[J].玉米科学,2018,26(5):30-36. |
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| 作者姓名： | 王琳琳 赵永平 张从省 杨海龙 王宏伟 谢传晓 |
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| 作者单位： | 沈阳农业大学特种玉米研究所;中国农业科学院作物科学研究所;中国农业科学院生物技术研究所;安徽农业大学生命科学院 |
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| 基金项目： | 国家科技支撑计划（2014BAD01B01）、"十三五"国家重点研发计划（2017YFD0300703） |
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| 摘 要： | 基于HiSeq高通量测序技术并结合生物信息学方法对X178、掖478两种材料授粉前后两个时期穗已知miRNA及靶基因进行分析。结果发现,X178授粉前后差异表达miRNA有100个,分别属于21个miRNA家族;掖478授粉前后共筛选出98个有差异的miRNA,分别属于22个miRNA家族。利用miRNA与靶基因mRNA负调控关系初步确定授粉前后miRNA可能调控的mRNA靶点,建立miRNA-靶mRNA一一对应关系。结果表明,X178中上调表达的13个miRNA对应11个下调表达靶基因,下调表达的33个miRNA对应41个上调表达靶基因;掖478中上调表达的5个miRNA对应7个下调表达靶基因,下调表达的28个miRNA对应33个上调表达靶基因,其中,6个miRNA家族共靶向11种mRNA。研究结果表明,在授粉前后玉米穗中已知miRNA在不同玉米自交系中种类具有差异。
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| 关 键 词： | 玉米 microRNA 深度测序 RNA seq 差异分析 |
| 收稿时间： | 2018/1/13 0:00:00 |
Differential Analysis of miRNA Expression Before and After Pollination in Maize Inbred Lines X178 and Ye478 |
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| WANG Lin-lin,ZHAO Yong-ping,ZHANG Cong-sheng,YANG Hai-long,WANG Hong-wei and XIE Chuan-xiao.Differential Analysis of miRNA Expression Before and After Pollination in Maize Inbred Lines X178 and Ye478[J].Journal of Maize Sciences,2018,26(5):30-36. |
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| Authors: | WANG Lin-lin ZHAO Yong-ping ZHANG Cong-sheng YANG Hai-long WANG Hong-wei and XIE Chuan-xiao |
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| Institution: | Special Corn Institute of Shenyang Agricultural Science, Shenyang 110866;Institute of Biotechnology, China Academy of Agricultural Sciences, Beijing 100081,School of Life Sciences, Anhui Agricultural University, Hefei 230036,Institute of Biotechnology, China Academy of Agricultural Sciences, Beijing 100081;Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China,Institute of Biotechnology, China Academy of Agricultural Sciences, Beijing 100081,Special Corn Institute of Shenyang Agricultural Science, Shenyang 110866 and Institute of Biotechnology, China Academy of Agricultural Sciences, Beijing 100081 |
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| Abstract: | Combined with bioinformatic method, the target genes of known miRNA in X178 and Ye478 were analyzed by HiSeq high-throughput sequencing technique with two periods of the ear before and after pollination. Results indicated that 100 miRNAs of X278 before and after pollination were different expression, which were attributed to 21 miRNAs families and 98 miRNAs of Ye478 before and after pollination were different expression, which were attributed to 22 miRNAs families. By using the negative regulation relationship between miRNA and target mRNA to preliminary determinate miRNA possible regulatory mRNA target before and after pollination, then we establish correspondence between miRNA-target mRNA. Results revealed that 13 miRNAs up-regulation expressed correspond to 11 down-regulation expression target genes and 33 miRNAs down-regulation expressed correspond to 41 up-regulation expression target genes in X178. Five miRNAs up-regulation expressed correspond to 7 down-regulation expression target genes and 28 miRNAs down-regulation expressed correspond to 33 up-regulation expression target genes in Ye478, including 6 miRNAs families target to 11 mRNA. The results showed that the known miRNA of ear before and after pollination had different kinds in different maize inbred lines. |
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| Keywords: | Maize MicroRNA Deep sequencing RNA seq Difference analysis |
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