| 兔葡萄球菌的分离与鉴定 |
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| 引用本文： | 王孝友,王永康,杨睿,付利芝,沈克飞,李成洪,杨松全,黄伟.兔葡萄球菌的分离与鉴定[J].黑龙江畜牧兽医,2012(13):99-101. |
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| 作者姓名： | 王孝友 王永康 杨睿 付利芝 沈克飞 李成洪 杨松全 黄伟 |
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| 作者单位： | 重庆市畜牧科学院;重庆市畜牧技术推广总站;西南大学荣昌校区 |
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| 基金项目： | “十一五”国家科技支撑计划项目“三峡库区肉兔健康养殖关键技术集成与示范”(2011BAD36B03);重庆市科委攻关项目“优质肉兔良种繁育、健康养殖及加工关键技术研究”(CSTC 2008AB1123) |
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| 摘 要： | 为了确定重庆市某兔场洪灾后母兔发生化脓性疾病的病原及筛选敏感的药物,试验进行了细菌分离培养、染色镜检、生化试验、血浆凝固酶试验、动物接种试验、16S rRNA基因扩增及测序、药物敏感试验。结果表明:分离的病原菌是金黄色葡萄球菌,其基因序列与金黄色葡萄球菌的同源性高达99%;分离菌对氨苄西林、先锋Ⅴ、菌必治、氨苄西林/舒巴坦高度敏感。
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| 关 键 词： | 兔 葡萄球菌 分离 鉴定 |
Isolation and identification of rabbit Staphylococcus spp. |
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| WANG Xiao-you,WANG Yong-kang,YANG Rui,FU Li-zhi,SHEN Ke-fei,LI Cheng-hong,YANG Song-quan,HUANG Wei.Isolation and identification of rabbit Staphylococcus spp.[J].Heilongjiang Animal Science And veterinary Medicine,2012(13):99-101. |
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| Authors: | WANG Xiao-you WANG Yong-kang YANG Rui FU Li-zhi SHEN Ke-fei LI Cheng-hong YANG Song-quan HUANG Wei |
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| Institution: | 1.Chongqing Academy of Animal Sciences,Chongqing 402460,China;2.Chongqing Animal Husbandry Techniques Extension Center,Chongqing 401121,China;3.Rongchang Campus, South-west Agriculture University,Chongqing 402460,China) |
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| Abstract: | To determine the pathogens causing suppurative diseases in adult female rabbits from in a rabbit farm in Chongqing after a flood,and to screen the sensitive drugs against the diseases.The pathogenic diagnosis were carried out using the bacterial culture and isolation,staining microscopy,biochemical test,plasma coagulase test,the animal inoculation test,16S rRNA amplification and sequencing,and drug sensitivity test.The results showed that the sequence homology of the pathogenic microbe had high similarity(99%) to Staphylococcus aureus,and the isolated pathogenic bacteria was identified as Staphylococcus aureus.The pathogenic bacteria was highly sensitive to ampicillin,cefalexin,ceftriaxone and ampicillin-sulbactam. |
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| Keywords: | rabbit Staphylococcus spp isolation identification |
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