| Boer山羊与山西地方山羊品种聚类分析 |
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| 引用本文： | 李步高,杜美红,郭晓红,曹果清,刘建华,周忠孝.Boer山羊与山西地方山羊品种聚类分析[J].山西农业大学学报(自然科学版),2005,25(3):243-244,263. |
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| 作者姓名： | 李步高 杜美红 郭晓红 曹果清 刘建华 周忠孝 |
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| 作者单位： | 1. 山西农业大学,动物科技学院,山西,太谷,030801
2. 中国农业大学,生物学院,北京,100094 |
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| 基金项目： | 山西省青年基金资助课题(2001041) |
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| 摘 要： | 应用RAPD技术对Boer山羊与山西地方山羊品种的遗传多态性进行了研究,利用基因频率进行了聚类分析,结果表明:1)四个品种的RAPD标记具有丰富的多态性,多态性带占总带数的63.81%,单个引物获得RAPD带数在3～10之间,各片段分子量大小在200～3252bp之间,所得多态性位点频率的变化范围在50%～81.8%。2)聚类分析表明:黎城大青羊与阳城白山羊首先聚为一类,具有较近的亲缘关系,吕梁黑山羊具有独特的遗传特性。山西主要地方山羊聚为一类,与国外品种波尔山羊亲缘关系较远。3)根据对4个品种遗传差异的分析,认为波尔山羊与吕梁黑山羊进行杂交组合,可望获得较高的杂种优势。
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| 关 键 词： | RAPD 山羊 多态性 杂种优势 |
| 文章编号： | 1671-8151(2005)03-0243-03 |
Cluster Analysis on Boer and Shanxi Native Goats |
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| LI Bu-gao et al..Cluster Analysis on Boer and Shanxi Native Goats[J].Journal of Shanxi Agricultural University,2005,25(3):243-244,263. |
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| Authors: | LI Bu-gao |
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| Abstract: | Genetic polymorphism and cluster analysis on Boer and Shanxi native goats (Yangcheng White、Licheng Daqing、Luliang Black) werestudied using randomly amplified polymorphic DNA (RAPD) technique, the results of this study indicatedas follows:1)The DNA of 4 breeds provideda great number of molecular markers with high polymorphism. One hundred and five loci were generated, 67 loci (63.81%) of which were highly polymorphic and 38 loci were commonfor all samples. Every single primer generated three to ten RAPD bands and all amplified DNA fragments in length were between 200~3252 bp withpolymorphic frequency from 50%~81.8%.2)Cluster analysis showed that the Shanxi native goats had a larger genetic distance with Boer, Licheng Daqing and Yangcheng White from alike region had a smaller distance. Luliang Black had its special genetic characteristics.3 ) According to the theory of heterosis and analysis on the genetic diversity of 4 breeds, the higher heterosis may be gained in cross combination betweenBoer and Luliang Black. |
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| Keywords: | RAPD Goats Polymorphic Heterosis |
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