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Residues of diflubenzuron on horse chestnut (Aesculus hippocastanum) leaves and their efficacy against the horse chestnut leafminer, Cameraria ohridella
Authors:Nejmanová Jana  Cvacka Josef  Hrdý Ivan  Kuldová Jelena  Mertelík Josef  Muck Alexander  Nesnerová Petra  Svatos Ales
Institution:Department of Natural Products, Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo Nám. 2, CZ-166 10 Praha 6, Czech Republic.
Abstract:Residues of the insect growth regulator diflubenzuron were quantified on horse chestnut (Aesculus hippocastanum L.) leaves treated with a diflubenzuron 480 g litre?1 SC, Dimilin. To analyse the samples, an analytical procedure was developed involving a simple extraction step followed by high‐performance liquid chromatography on an octadecyl‐modified silica column with methanol + 0.01 M ammonium acetate mobile phase. The results showed diflubenzuron to be highly stable on horse chestnut leaves; more than 4 months (127 days) after application, 38% (on average) of the insecticide still remained on/in the leaves. The data confirmed biological observations showing diflubenzuron's long‐term efficacy against the horse chestnut leafminer, Cameraria ohridella Deschka & Dimi?, which is the most important pest of the horse chestnut in Europe. The hypothesis of possible penetration of diflubenzuron into the leaf mass is explored and discussed. Copyright © 2006 Society of Chemical Industry
Keywords:leafminer  Cameraria ohridella  horse chestnut  Aesculus hippocastanum  liquid chromatography  persistence  pesticide
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