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| 地黄RgPR-10基因的克隆与表达 | |
| 引用本文： | 孙鹏,郭玉海,祁建军,周丽莉,张苗苗,李先恩.地黄RgPR-10基因的克隆与表达[J].西北农业学报,2009,18(1):300-304. |
| 作者姓名： | 孙鹏 郭玉海 祁建军 周丽莉 张苗苗 李先恩 |
| 作者单位： | 1. 中国医学科学院,药用植物研究所,北京,100193;中国农业大学,农学与生物技术学院,北京,100193 2. 中国农业大学,农学与生物技术学院,北京,100193 3. 中国医学科学院,药用植物研究所,北京,100193 |
| 基金项目： | 国家自然科学基金，北京市自然科学基金 |
| 摘 要： | 为研究地黄块根的发育机制,通过抑制消减杂交方法从地黄块根中克隆到一个多拷贝、含有完整阅读框编码154个氨基酸的RgPR-10基因(GenBank登录号为EU526395).表达分析发现地黄RgPR-10基因主要在根、茎中表达,尤其是块根中具有较高的表达量.序列分析发现该基因序列与其他物种相似性较低,可能具有不同的功能.为进一步研究该基因在地黄块根发育中的作用,对该基因进行了原核表达,并对融合蛋白进行了初步纯化,电泳检测纯化蛋白具有较高的纯度,可以用于下一步制备抗体和生化功能分析. |
| 关 键 词： | 抑制消减杂交 地黄 原核表达 |
| 收稿时间： | 2008/3/14 0:00:00 |
| 修稿时间： | 2008/8/12 0:00:00 |
Clonning and Expression of RgPR-10 Gene from Rehmannia glutinosa |
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| SUN Peng,GUO Yuhai,QI Jianjun,ZHOU Lili,ZHANG Miaomiao and LI Xianen.Clonning and Expression of RgPR-10 Gene from Rehmannia glutinosa[J].Acta Agriculturae Boreali-occidentalis Sinica,2009,18(1):300-304. | |
| Authors: | SUN Peng GUO Yuhai QI Jianjun ZHOU Lili ZHANG Miaomiao and LI Xianen |
| Institution: | The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China;College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China;College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China;The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China;The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China;The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China;The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China |
| Abstract: | In order to investigate the storage root development mechanism of Rehmannia glutinosa, SSH(suppression subtractive hybridization)was performed to isolate storage root development related genes.One high copy RgPR-10 gene(GenBank,Accession No.EU 526395)with complete open reading frame which encoded 154 amino acids was isolated from the SSHlibrary.The RgPR-10 gene of Rehmannia glutinosa shared low identity with other species.Northern blotting analysis showed that RgPR-10 was ex pressed mainly in root and stem and accumulated in storage root at high level.In order to further investigate its functions in Rehmannia glutinosa,the RgPR-10 gene was expressed in E.coli BL 21 and purified by gel purification method.The purity of the purified protein is relative high and can be used for further study. |
| Keywords: | PR-10 |
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