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粗山羊草高分子量谷蛋白亚基分析
引用本文:闫贵云,刘少翔,孙玉,孙善澄.粗山羊草高分子量谷蛋白亚基分析[J].山西农业科学,2008,36(11).
作者姓名:闫贵云  刘少翔  孙玉  孙善澄
作者单位:山西省农业科学院作物遗传研究所,山西,太原,030031
基金项目:山西省农业科学院青年基金 
摘    要:粗山羊草中含有丰富的高分子量谷蛋白亚基的变异类型,山羊草属中的山羊草组(2n=14,DD)是普通小麦(2n=42,AABBDD)D染色体组的供体。并且1D染色体上编码的HMW-GS与小麦烘烤品质最为密切。与普通小麦G lu-D1位点相似,粗山羊草1D染色体上也编码了迁移率不同的两个HMW-GS,但粗山羊草不同种间存在更丰富的HMW-GS变异类型。现在粗山羊草中所发现的HMW-GS远高于小麦,粗山羊草中的亚基类型除了2+12,5+10常见于普通小麦,5+12亚基仅存在于意大利个别小麦品种外,其余为粗山羊草所特有的亚基类型。对粗山羊草进行SDS-PAGE凝胶电泳,并对所提供的粗山羊草的HMW-GS进行鉴定分析,得到一些粗山羊草中所特有的优质亚基类型,其中有目前认为更为优秀的1.5+10亚基,为创造新的人工合成六倍体小麦提供了材料基础。

关 键 词:粗山羊草  小麦  高分子量谷蛋白亚基

Analysis to High-molecular-weight Glutenin Subunits from Aegilops tauschii
YAN Gui-yun,LIU Shao-xiang,SUN Yu,SUN Shan-cheng.Analysis to High-molecular-weight Glutenin Subunits from Aegilops tauschii[J].Journal of Shanxi Agricultural Sciences,2008,36(11).
Authors:YAN Gui-yun  LIU Shao-xiang  SUN Yu  SUN Shan-cheng
Abstract:The Aegilops tauschii includes rich variation types of HMW-GS,Aegilops tauschii,belonging to the Aegilops tauschii group(2n=14,DD) and it is the D-genome donor of Triticum aestivum(2n=42,AABBDD).HMW-GS encoded in the 1D chromosome is the closest to wheat baking quality.Similaring with the triticum aestivum Glu-D1 positionspot,the Aegilops tauschii 1D dyeing raises also codes transportratio different two HMW-GS,but in Aegilops tauschii differentinter-species there exists richer HMW-GS variation type.Now HMW-GS discovered in the Aegilops tauschii center is far higher than in the wheat and the Aegilops tauschii center Asian base type except 2 12,5 10,common in Triticum aestivum,5 12 Asian base only exists in individual Italian wheat variety,the rest for Aegilops tauschii are unique Asia base type.In this experiment,Aegilops tauschii HMW-GS were carried on the SDS-PAGE gelatin electricity and obtained some Aegilops tauschii center unique high quality Asia base types,including the most outstanding 1.5 10 Asian base at present,which provided material foundation for created the new manual synthesissixfold body wheat.
Keywords:Aegilops tauschii  Triticum aestivum  High-molecular-weight glutenin subunits
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