| 用分光光度计预测小球藻藻液浓度的研究 |
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| 引用本文： | 潘竞洋,;赵之旭,;吴良柏,;张伟,;徐俊生,;陈玉桂,;付志德.用分光光度计预测小球藻藻液浓度的研究[J].安徽农业科学,2014(25):8504-8505. |
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| 作者姓名： | 潘竞洋 ;赵之旭 ;吴良柏 ;张伟 ;徐俊生 ;陈玉桂 ;付志德 |
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| 作者单位： | [1]国家林业局甘肃濒危动物保护中心,甘肃武威733000; [2]钱学森沙产业中心实验室,甘肃武威733000; [3]清华大学航天航空学院,北京100084 |
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| 基金项目： | 甘肃省科技厅星火计划项目（5HS066-A91-015-01）.鸣谢 本论文研究过程中得到清华大学宋耀祖教授的指导. |
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| 摘 要： | 目的]为实现小球藻大面积推广生产和产业化发展提供科学、翔实的技术参数。方法]通过721型分光光度计,对不同浓度的小球藻藻液在不同波段光照下的透光度进行测定,确定测定小球藻的最佳照射波段,得到最佳照射波段时小球藻藻液的浓度。结果]在不同光照对不同浓度小球藻藻液透射的吸光度进行归一化处理后,得出小球藻的最佳透射波段为670 nm。同时,得出670 nm光照下吸光度与小球藻浓度的回归方程为:y=-1.479 7+0.987x,相关系数为0.991,说明相关性较好。推导了当量浓度(C)与透光度(I)的公式,C=-ln(I/I0)/(LK),式中L为光径长度(比色皿宽度),K为小球藻细胞的平均截面面积。结论]用分光光度计间接测量小球藻的浓度是可行的。
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| 关 键 词： | 小球藻 最佳波段 当量浓度 |
Concentration Prediction of Chlorella vulgaris Beij by Visible Spectrophotometer |
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| Institution: | PAN Jing-yang,ZHAO Zhi-xu(1.Gansu Endangered Animal Protection Centre of the State Forestry Administration, Wuwei, Gansu 733000 ;2.Qian Xuesen Deserticulture Central Laboratory, Wuwei, Gansu 733000) |
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| Abstract: | Objective] The research aimed to provide the scientific and detailed technical parameters for the achievement of massive popularization and industrialization development of Chlorella vulgaris Beij.Method] Different concentrations of Chlorella vulgaris Beij were tested to get the light absorbance of algae by 721 Visible Spectrophotometer under 41 groups of wave bands.So the optimal wave band used to produce Chlorella could be checked out.Result] The optimal wave band was 670 nm.This was due to the transmitted light intensity of algae got to the peak under 670 nm of incident light.The relationship between the light absorbance and the concentration of algae was y =-1.479 7 ＋ 0.987x.The relativity between them equaled 0.991.It showed that they had good relativity.The formulate of the algae concentration （C） and the transmitted light intensity （Ⅰ） was C =-In （I/I0）/（LK）.L was the width of the vessel,and K was the section area of an algae cell.Conclusion] The indirect measurement of Chlorella vulgaris Beij by Visible Spectrophotometer was feasible. |
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| Keywords: | Chlorella Optimal wave bands Concentration |
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