| 根癌农杆菌介导的甘蔗遗传转化体系的研究 |
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| 引用本文： | 李瑞美,郑回勇,陈永快,吴水金,鞠玉栋,吴松海.根癌农杆菌介导的甘蔗遗传转化体系的研究[J].江西农业学报,2006,18(2):22-25. |
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| 作者姓名： | 李瑞美 郑回勇 陈永快 吴水金 鞠玉栋 吴松海 |
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| 作者单位： | 福建省农业科学院,甘蔗研究所,福建,漳州,363005 |
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| 基金项目： | 福建省自然科学基金计划资助项目(项目编号:Z0516053) |
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| 摘 要： | 采用根癌农杆菌介导法将苏云金氏芽孢杆菌毒蛋白B.t基因cryIA转入甘蔗胚性愈伤组织中,建立农杆菌转化甘蔗的遗传转化体系,获得13棵再生植株。经GUS及PCR检测,cryIA确实已转移到甘蔗幼苗中,GUS在再生植株中也得到瞬时表达。
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| 关 键 词： | 甘蔗 根癌农杆菌介导 遗传转化 |
| 文章编号： | 1001-8581（2006）02-0022-04 |
| 收稿时间： | 2006-01-09 |
| 修稿时间： | 2006年1月9日 |
Study on Genetic Transformation of Sugarcane Mediated by Agrobacterium tumefaciens |
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| LI Rui-mei,ZHENG Hui-yong,CHEN Yong-kuai,WU Shui-jin,JU Yu-dong,WU Song-hai.Study on Genetic Transformation of Sugarcane Mediated by Agrobacterium tumefaciens[J].Acta Agriculturae Jiangxi,2006,18(2):22-25. |
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| Authors: | LI Rui-mei ZHENG Hui-yong CHEN Yong-kuai WU Shui-jin JU Yu-dong WU Song-hai |
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| Institution: | Institute of Sugarcane, Fujian Academy of Agricultural Sciences, Zhangzhou 363005, China |
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| Abstract: | It introduced B.t gene cryIA to sugarcane callus by Agrobacterium tumefaciens.An optimum protocol of transformation was established and 13 regenerated seedlings were obtained from transformants.GUS and PCR test of seedlings confirmed that cryIA has been transferred to sugarcane.It also detected the transient expression of GUS gene. |
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| Keywords: | Sugarcane Agrobacterium tumefaciens-mediated Genetic transformation |
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