| 不同培养条件对金铁锁毛状根生长的影响 |
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| 引用本文： | 王洪峰.不同培养条件对金铁锁毛状根生长的影响[J].广东林业科技,2012,28(2):16-21. |
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| 作者姓名： | 王洪峰 |
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| 作者单位： | 广东省林业科学研究院 |
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| 基金项目： | 林业公益性行业专项“华南立体林业经营模式研究与示范”（项目任务书编号：201004047）。 |
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| 摘 要： | 利用发根农杆菌ATCC15834诱导金铁锁获得毛状根,研究金铁锁毛状根的最优培养条件。结果表明,在5种培养基中,以在1/2MS培养基中皂苷的总产量最大;添加3%蔗糖、水解乳蛋白(LH)及IBA利于毛状根的生长及总皂苷的积累;而添加IAA、NAA、6-BA及蛋白胨抑制其生长。
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| 关 键 词： | 金铁锁 毛状根 培养 皂苷 |
| 收稿时间： | 2011/11/25 0:00:00 |
| 修稿时间： | 2/21/2012 3:16:33 PM |
Effects of Culture Conditions on the Hairy Roots Growth of Psammosilene tunicoides |
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| Wang Hongfeng.Effects of Culture Conditions on the Hairy Roots Growth of Psammosilene tunicoides[J].Forestry Science and Technology of Guangdong Province,2012,28(2):16-21. |
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| Authors: | Wang Hongfeng |
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| Institution: | Gao Shuai Wang Hongfeng Hou Lili Zhang Hong Xu Liang( 1. Guangzhou University of Chinese Medicine, Guangzhou, 510520 ; 2. Guangdong Academy of Forestry) |
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| Abstract: | Hairy root can be induced from Psammosilene tunicoides by Agrobacterium rhizogenes ATCC15834. The effects of different culture conditions on hairy roots growth were studied. Among five media used, the medium 1/2 MS was the best one for the saponins content. On the source concentration concerned, 3% sucrose concentration could increase the hairy root growth. The hydrolytic lactalbumin and IBA could improve the hairy root growth and total saponins production. IAA. NAA. 6-BA and peptone could inhibit hairy root growth. |
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| Keywords: | Psammosilene tunicoides hairy roots culture saponins |
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