| 利用siRNA高通量测序技术检测烟草病毒 |
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| 引用本文： | 王芳,周本国,许大凤,高正良.利用siRNA高通量测序技术检测烟草病毒[J].植物病理学报,2015,45(1):97-100. |
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| 作者姓名： | 王芳 周本国 许大凤 高正良 |
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| 作者单位： | 安徽省农业科学院烟草研究所, 合肥 230031 |
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| 基金项目： | 安徽省烟草公司科技攻关项目“安徽省烟草有害生物调查研究”(20100551005);公益性行业(农业)科研专项经费资助(201203091) |
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| 摘 要： | <正>烟草是我国的主要经济作物之一,病毒病是烟草生产上的重要病害。常见的烟草病毒主要有马铃薯Y病毒(Potato virus Y,PVY)、黄瓜花叶病毒(Cucumber mosaic virus,CMV)、烟草花叶病毒(Tobacco mosaic virus,TMV)、烟草脉带花叶病毒(Tobacco vein banding mosaic virus,TVMBV)等1]。此外,近年来在烟草上还发生一些新的病毒病害,如南美红辣椒脉斑驳病毒(Chilli veinal mottle virus,ChiVMV)2]、番茄环纹斑点病毒(Tomato zonate spot virus,ZTSV)3]和番茄斑萎病毒(Tomato spotted wilt virus,TSWV)4]。这些病毒
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| 关 键 词： | high-throughput sequencing small interference RNA detection virus |
| 收稿时间： | 2014-02-04 |
Detection for RNA viruses in tobacco by High-throughput sequencing of siRNA |
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| WANG Fang,ZHOU Ben-guo,XU Da-feng,GAO Zheng-liang.Detection for RNA viruses in tobacco by High-throughput sequencing of siRNA[J].Acta Phytopathologica Sinica,2015,45(1):97-100. |
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| Authors: | WANG Fang ZHOU Ben-guo XU Da-feng GAO Zheng-liang |
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| Institution: | Tobacco Institute, Anhui Academy of Agricultu-ral Sciences, Hefei 230031, China |
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| Abstract: | Plant obtains immunity against RNA virus through small interference RNA (siRNA) mechanism. we adopt next-generation sequencing technology to perform high throughput sequencing of tabacco siRNA and screening for RNA virus sequences with bioinformatics methods. We found that PVY, CMV, TMV, TVBMV and other virus genomic information in Anhui Province. This research established a new strategy to find naturally RNA viruses in tobacco, and this strategy could be applied to virus surveillance in tobacco. |
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| Keywords: | high-throughput sequencing small interference RNA detection virus |
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