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| 利用小RNA深度测序和组装技术鉴定紫藤花叶病病原 | |
| 引用本文： | 苏秀,徐毅,陈莎,傅帅,钱亚娟,张立钦,周雪平.利用小RNA深度测序和组装技术鉴定紫藤花叶病病原[J].植物病理学报,2015,45(1):88-92. |
| 作者姓名： | 苏秀 徐毅 陈莎 傅帅 钱亚娟 张立钦 周雪平 |
| 作者单位： | 1.浙江大学生物技术研究所,杭州 310058; 2.浙江农林大学亚热带森林培育国家重点实验室培育基地,临安 311300 |
| 基金项目： | 质检公益性行业科研专项项目(201310068); 浙江省重中之重林学一级学科开放基金项目(KF201330); 浙江农林大学科研发展基金项目(2013FK019) |
| 摘 要： | <正>RNA沉默(RNA silencing)是一种在真核生物体内普遍保守的基于核酸序列特异性抑制基因表达的调控机制1]。2009年Kreuze等2]发现病毒特异的小RNA(small RNA,sRNA)在序列上是重叠的,因此推测通过深度测序技术获得的大量sRNA序列能用来组装病毒的基因组并用来鉴定和发现新病毒。利用sRNA深度测序技术已在作物和昆虫上鉴定发现多种病毒3、4],但在木本植物上还未见报道。 |
| 关 键 词： | small RNA Wisteria vein mosaic virus deep sequencing |
| 收稿时间： | 2014-03-01 |
Detection of viruses infecting Wisteria sinensis by deep sequencing and assembly of small RNA |
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| SU Xiu,XU Yi,CHEN Sha,FU Shuai,QIAN Ya-juan,ZHANG Li-qin,ZHOU Xue-ping.Detection of viruses infecting Wisteria sinensis by deep sequencing and assembly of small RNA[J].Acta Phytopathologica Sinica,2015,45(1):88-92. | |
| Authors: | SU Xiu XU Yi CHEN Sha FU Shuai QIAN Ya-juan ZHANG Li-qin ZHOU Xue-ping |
| Institution: | 1 Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China; 2 The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry University, Lin’an 311300, China |
| Abstract: | Plant defense against viruses through small RNA (sRNA) mediated RNA interference mechanism. Analysis of virus derived sRNA profiles in plant can be applied for de novo assembly of virus genomes and virus identification. In this study, suspected virus-infected Wisteria sinensis samples collected from Zijingang Campus of Zhejiang University were used for sRNA library construction and deep sequencing. After assembly of total sRNAs, it was found that W. sinensis leaves were infected by Wisteria vein mosaic virus (WVMV). The library generated 18.9 million sRNA reads, of which 0.32 million were WVMV-derived sRNAs. Using de novo assembly, 23.3% of full length genome nucleotide sequence of a previously reported potyvirus WVMV was obtained. To confirm the existence of WVMV in the samples, WVMV coat protein (CP) gene sequence was obtained by RT-PCR, and ensured by Sanger sequencing. Taken together, the data suggest that sRNA deep sequencing technology is an efficient and powerful genetic tool for virus identification in woody plants. |
| Keywords: | small RNA Wisteria vein mosaic virus |
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