Identification of synthetic regioisomeric lutein esters and their quantification in a commercial lutein supplement |
| |
| Authors: | Young J Christopher Abdel-Aal El-Sayed M Rabalski Iwona Blackwell Barbara A |
| |
| Institution: | Food Research Program, Agriculture & Agri-Food Canada, 93 Stone Road West, Guelph, Ontario, Canada. youngjc@agr.gc.ca |
| |
| Abstract: | Synthetic mixtures of 24 mono- and diesters of the asymmetric hydroxylated carotenoid lutein with lauric, myristic, palmitic, and stearic acids were analyzed by liquid chromatography-ultraviolet/visible spectroscopy (LC-UV-vis) and characterized by LC-mass spectrometry (MS) and nuclear magnetic resonance spectroscopy (NMR). These compounds were then used for identifying the composition of a commercial lutein supplement. This is the first report of chromatographic separation of mixed fatty acid lutein diesters. Preferential MS loss of fatty acids or water occurred initially at the 3'-hydroxy position in the epsilon-ionone ring and subsequently at the 3-hydroxy position in the beta-ionone ring. This selective fragmentation leads to facile assignment of the specific fatty acids to the appropriate regioisomeric ionone ring. A commercial lutein supplement contained low levels of two pairs of regioisomeric monoesters and nearly equal levels of three homogeneous diesters and five pairs of mixed diesters. Palmitic acid was the predominant fatty acid, with lower amounts of myristic, stearic, and lauric acids. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
