Screening of Chinese cabbage mutants produced by 60Co γ‐ray mutagenesis of isolated microspore cultures |
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| Authors: | Danyang Li Runpeng Yao Qian Meng Hui Feng |
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| Institution: | Department of Horticulture, Shenyang Agricultural University, , Shenyang, 110866 China |
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| Abstract: | Since the release of the Chinese cabbage genome sequence, increasing interest has focused on the functional analysis of unidentified genes in Chinese cabbage. Mutant analysis forms the basis of functional genomics research. To produce a variety of Chinese cabbage mutants in the same genetic background, buds containing late uninucleate spores from a doubled haploid line of the Chinese cabbage variety ‘Fukuda 50’ were irradiated with 60Co γ‐rays at doses of 20, 40 and 60 Gy. Then, the treated microspores were isolated and cultured. A total of 492 putative M0 mutants were isolated from 1483 regenerated plants. Of these, six M1 mutants were verified; the mutant frequency was 0.41%. These mutants comprise a mutant library that includes one plant shape mutant, two flower mutants and three male sterile mutants. Pollen viability detection and DNA flow cytometry were used to determine the ploidy of the regenerated plants. Some of the mutants isolated in this study may be useful for Chinese cabbage breeding and functional genomics research. |
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| Keywords: | Chinese cabbage mutagenesis γ ‐rays microspore culture mutant |
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