| 根癌农杆菌介导的香蕉枯萎病菌4号生理小种的转化 |
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| 引用本文： | 李敏慧,张荣,姜大刚,习平根,庄楚雄,姜子德.根癌农杆菌介导的香蕉枯萎病菌4号生理小种的转化[J].植物病理学报,2009,39(4):405-412. |
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| 作者姓名： | 李敏慧 张荣 姜大刚 习平根 庄楚雄 姜子德 |
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| 作者单位： | 1 华南农业大学资源与环境学院, 广州 510642;2 华南农业大学园艺学院, 广州 510642;3 华南农业大学生命科学院, 广州 510642 |
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| 基金项目： | 国家自然科学基金，广东省自然科学基金 |
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| 摘 要： | 本文针对香蕉枯萎病菌4号生理小种这一对我国香蕉生产构成了极大威胁的检疫性有害生物,建立了根癌农杆菌介导的该生理小种的转化体系,确定了影响转化效率主要因子的最佳条件分别是:共培养乙酰丁香酮(AS)浓度为200μmol/L、共培养时间为48 h、培养温度为25℃、诱导培养基pH值为5.5。此条件下,转化效率达到21~24个转化子/104香蕉枯萎病菌孢子。PCR和Southern杂交分析表明外源的T-DNA已经成功随机地整合到该病原菌基因组中,且多为单拷贝。应用该转化体系已获得近25 000个转化子,为研究该生理小种致病机制奠定了基础。
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| 关 键 词： | 香蕉枯萎病菌4号生理小种 农杆菌介导的转化 影响因子优化 |
Agrobacterium tumefaciens-mediated transformation of Fusarium oxysporum f.sp. cubense race 4 |
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| LI Min-hui,ZHANG Rong,JIANG Da-gang,XI Ping-gen,ZHUANG Chu-xiong,JIANG Zi-de.Agrobacterium tumefaciens-mediated transformation of Fusarium oxysporum f.sp. cubense race 4[J].Acta Phytopathologica Sinica,2009,39(4):405-412. |
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| Authors: | LI Min-hui ZHANG Rong JIANG Da-gang XI Ping-gen ZHUANG Chu-xiong JIANG Zi-de |
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| Institution: | 1 College of Natural Resources and Environment, South China Agricultural University, Guang-zhou 510642, China;2 College of Life Science, South China Agricultural University, Guangzhou 510642, China;3 College of Horticulture, South China Agricultural University, Guangzhou 510642, China |
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| Abstract: | Fusarium oxysporum f. sp. cubense (FOC) race 4, the quarantine fungus caused a highly destructive vascular wilt disease of banana, had been transformed by using Agrobacterium tumefaciens-mediated transformation. The objective of the study was to optimize the transformation system to obtain T-DNA inserted-mutants for gene functional study. The key conditions included co-cultivation with 200 μmol/L acetosyringone for 48 h, induction cultivation at pH 5.5 and 25℃. Under the conditions, transformation efficiency was in the range of 21 -24 transformants per 10~4 FOC race 4 conidia. PCR and Southern blot analysis of the transformants showed that the T-DNA had been successfully integrated into genome of the fungus with the majority of single copy at random sites. A total of 25 000 transformants had been obtained in the study, which provided a foundation for better understuding molecular mechanism of FOC pathogenicity. |
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| Keywords: | Fusarium oxysporum f sp cubense(FOC) race 4 Agrobacterium tumefaciens-mediated transformation factor on transformation efficiency |
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