| 石参中黄酮类化合物含量的测定及抗氧化活性的研究 |
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| 引用本文： | 郭豫梅,郭豫杰,刘贵,季晓晖,闵锁田,田光辉.石参中黄酮类化合物含量的测定及抗氧化活性的研究[J].安徽农业科学,2010,38(1):168-169. |
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| 作者姓名： | 郭豫梅 郭豫杰 刘贵 季晓晖 闵锁田 田光辉 |
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| 作者单位： | 陕西理工学院化学与环境科学学院,陕西汉中,723001;河南农业大学牧医工程学院,河南郑州,450002 |
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| 基金项目： | 陕西理工学院专项科研基金项目(SLGQD0711) |
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| 摘 要： | 目的]测定石参中总黄酮含量并对其抗氧化性进行研究。方法]采用超声波提取技术用体积分数为80％的乙醇提取石参中的总黄酮，分光光度法测定石参中的总黄酮类含量，研究石参中黄酮类化合物对超氧自由基、羟基自由基的清除作用。结果]石参中总黄酮含量为0．7425％，石参中黄酮类化合物对自由基清除作用随着黄酮浓度的增大而表现出明显的量效关系。总黄酮浓度为9．791×10^-4mg／ml时对超氧自由基的清除率最高，为48．91％；浓度为0．0099mg／ml时对羟基自由基的清除率最高，达到83．76％。结论]石参总黄酮对超氧自由基、羟基自由基均具有一定的清除作用。
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| 关 键 词： | 石参 黄酮类化合物 抗氧化活性 |
Determination of Total Flavonoids in Eremurus chinensis and Study on Its Antioxidant Activity |
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| GUO Yu-mei et al.Determination of Total Flavonoids in Eremurus chinensis and Study on Its Antioxidant Activity[J].Journal of Anhui Agricultural Sciences,2010,38(1):168-169. |
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| Authors: | GUO Yu-mei |
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| Institution: | School of Chemistry and Enviromental Sciences;Shaanxi University of Technology;Hanzhong;Shaanxi 723001 |
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| Abstract: | Objective] The aim was to determine total flavonoids in Eremurus chinens and study its antioxidant.Method] The total flavonoids in E.chinens were extracted by volume fraction of 80% ethanol and using ultrasonic extraction technology.The content was determined by spectrophotometric method.And the removal effect of flavonoids on superoxide radical,hydroxyl radical was studied.Result] The content of flavonoids in E.chinens was 0.742 5%.With the increasing of flavonoids,the removal effect on free radical bec... |
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| Keywords: | Eremurus chinensis Flavonoids Antioxidant activity |
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