| 口蹄疫病毒RT-PCR检测和分型方法的建立及初步应用 |
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| 引用本文： | 曲哲会,王君伟.口蹄疫病毒RT-PCR检测和分型方法的建立及初步应用[J].中国预防兽医学报,2007,29(4):316-319,322. |
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| 作者姓名： | 曲哲会 王君伟 |
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| 作者单位： | 东北农业大学,动物医学院,黑龙江,哈尔滨,150030 |
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| 摘 要： | 参考GenBank中各个血清型口蹄疫病毒3D、vp1、2A基因的标准序列，设计引物P1／P2和S1／S2。建立用于检测口蹄疫病毒及其利用引物S1／S2克隆片段同源性比较而确定血清型的RT-PCR方法。通过敏感性试验检测，2对引物均可以检测到10TCID50的病毒量；特异性试验的检测，2对引物对正常细胞、牛黏膜病病毒、猪瘟病毒、水疱性口炎病毒、牛传染性鼻气管炎病毒的检测结果均为阴性。利用该方法对病牛的流涎液体、水疱液体、舌皮组织、感染犊牛心脏等组织进行检测初步结果显示：该方法可以对O型和AsiaⅠ型口蹄疫病毒进行特异性检测，能够用于口蹄疫急性及亚临床感染的诊断及流行病学调查。
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| 关 键 词： | 口蹄疫病毒 分型 检测 |
| 文章编号： | 1008-0589（2007）04-0316-04 |
| 收稿时间： | 2006-05-18 |
| 修稿时间： | 2006年5月18日 |
Establishment and primary application of RT-PCR assay for detection and serotyping of foot-and-mouth disease virus |
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| QU Zhe-hui,WANG Jun-wei.Establishment and primary application of RT-PCR assay for detection and serotyping of foot-and-mouth disease virus[J].Chinese Journal of Preventive Veterinary Medicine,2007,29(4):316-319,322. |
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| Authors: | QU Zhe-hui WANG Jun-wei |
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| Abstract: | A RT-PCR method was developed for identification and differentiation of seven serotypes of (Foot-and-mouth disease virus,FMDV).Two pairs of primers P1/P2 and S1/S2 were designed based on the published nucleotide sequence of the 3D,1D and 2A genes of FMDV.The sensitivity of the assay was measured using Reed-Muench method.The results showed that the RT-PCR assay could detect 10 TCID_(50) of FMDV.Virus RNA could be detected in various tissues (heart,tongue,the spleen and so on) and the saliva of FMDV infected cows,and no cross reaction was detected for IBRS-2 cell,HCV,VSV, BVDV,IBRV.The results suggested that the RT-PCR is a specific and sensitive method,which could be used for detection and diagnosis of latent and subclinical FMDV O type. |
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| Keywords: | RT-PCR |
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