|
|||||
|
|
| 超滤—离子层析法精制猪硫酸软骨素 | |
| 引用本文： | 陆钊,董树国,王玥,曹宏梅,赖红伟,康永炜,董顺福.超滤—离子层析法精制猪硫酸软骨素[J].吉林农业大学学报,2012,34(2):171-175. |
| 作者姓名： | 陆钊 董树国 王玥 曹宏梅 赖红伟 康永炜 董顺福 |
| 作者单位： | 1. 吉林医药学院药学院,吉林,132013 2. 吉林石化分公司电石厂,吉林,132022 3. 吉林医药学院实验中心基础化学实验室,吉林,132013 |
| 摘 要： | 采用超滤—离子层析法精制猪硫酸软骨素,离子对反相高效液相色谱法测定其含量,红外吸收光谱法测定其结构.研究了超滤液温度、超滤压力、超滤液pH对猪硫酸软骨素精制时膜通量和得率的影响.结果表明:超滤—离子层析技术可应用于精制猪硫酸软骨素.猪硫酸软骨素超滤的工艺条件:以微孔滤膜处理原料液,超滤膜的截留分子量10 kD,超滤压力0.25MPa,超滤温度25℃,溶液pH为9.产物纯度为95.74％,产率为80.15％.该方法精确度和准确度都较高,可用于猪硫酸软骨素的精制. |
| 关 键 词： | 猪硫酸软骨素 超滤膜法 离子交换层析法 精制 |
Refinement of Porcine Chondroitin Sulfate by UF Membrane-ion-exchange Chromatography |
|
| LU Zhao , DONG Shu-guo , WANG Yue , CAO Hong-mei , LAI Hong-wei , KANG Yong-wei , DONG Shun-fu.Refinement of Porcine Chondroitin Sulfate by UF Membrane-ion-exchange Chromatography[J].Journal of Jilin Agricultural University,2012,34(2):171-175. | |
| Authors: | LU Zhao DONG Shu-guo WANG Yue CAO Hong-mei LAI Hong-wei KANG Yong-wei DONG Shun-fu |
| Institution: | 1.College of Pharmacy,Jilin Medical College,Jilin 132013,China;2.Calcium Carbide Factory of Petro China,Jilin Petrochemical Co.Ltd.,Jilin 132022,China;3.Basic Chemistry Laboratory of Experimental Center,Jilin Medical College,Jilin 132013,China |
| Abstract: | Porcine chondroitin sulfate was refined by UF membrane-ion-exchange-chromatography.Its structure was determined with infrared spectroscopy and its content was determined by Ion-pair RP—HPLC.The effects of ultrafiltrate temperature,ultrafiltration pressure and ultrafiltrate pH on the flux and yield of porcine chondroitin sulfate were studied when it was refined.UF membrane-ion-exchange chromatography can be used to refine porcine chondroitin sulfate.The process conditions of the refinement of porcine chondroitin sulfate: using microporous membrane to process raw material liquid,the molecular weight cutoff of UF membrane is 10 kD,ultrafiltrate pressure 0.25 MPa,ultrafiltrate temperature 25 ℃ and pH 9.Purity of products is 95.74% and yield 80.15%.This method is of high accuracy and can be used to refine porcine chondroitin sulfate. |
| Keywords: | porcine chondroitin sulfate ultrafiltrationmembrane process ion-exchange-chromatography refine |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |