| 李属植物叶片红色性状RAPD分子标记研究 |
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| 引用本文： | 温亮,王中华,霍雪梅,李彦慧,张晓曼,杨建民.李属植物叶片红色性状RAPD分子标记研究[J].河北农业大学学报,2009,32(4). |
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| 作者姓名： | 温亮 王中华 霍雪梅 李彦慧 张晓曼 杨建民 |
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| 作者单位： | 河北农业大学,园林与旅游学院,河北,保定,071001 |
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| 摘 要： | 以李属(Prunus)植物‘红叶李’(P.salicina×P.atropurupurea)和‘安哥诺李’(Prunus salicinacv.‘angenuo’)正反交的56株F1代群体为试材,结合分离群体分组分析法(Bulked Segregate Analysis,BSA)构建"红叶"和"绿叶"近等基因池,应用RAPD标记技术,进行了李属植物叶片红色性状相连锁的分子标记研究。通过对350个随机引物的筛选,获得一个在红叶基因池中能稳定扩增的RAPD标记,该片段大小约为2 300 bp。经过重复性验证和群体单株验证,该标记片段仅在红叶个体(重组型除去)中出现,表明与李属植物叶片红色性状相连锁。
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| 关 键 词： | 李属 叶色 红叶性状 RAPD |
RAPD markers linked to the red leaf traits of Prunus |
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| WEN Liang,WANG Zhong-hua,HUO Xue-mei,LI Yan-hui,ZHANG Xiao-man,YANG Jian-min.RAPD markers linked to the red leaf traits of Prunus[J].Journal of Agricultural University of Hebei,2009,32(4). |
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| Authors: | WEN Liang WANG Zhong-hua HUO Xue-mei LI Yan-hui ZHANG Xiao-man YANG Jian-min |
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| Institution: | WEN Liang,WANG Zhong-hua,HUO Xue-mei,LI Yan-hui,ZHANG Xiao-man,YANG Jian-min (College of L,scape Architecture , Tourism,Agricultural University of Hebei,Baoding 071001,China) |
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| Abstract: | In this study, a RAPD marker linked to red leaf traits was screened from 350 RAPD arbitrary primers in 56 F1 individuals from the cross P.salicina×P.atropurupurea and Prunus salicina cv. 'angenuo', leaf red /green pool was constructed respectively using RAPD technique and BSA method. After repeating tests and checking up individuals of population, this marker could only be amplified in the red leaf individuals (except recombinants). The size of this marker was about 2 300 bp. |
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| Keywords: | RAPD Prunus leaf color red leaf traits RAPD |
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