| 川渝地区奶牛隐性乳房炎调查和主要病原菌分离鉴定及药敏试验 |
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| 引用本文： | 王裕文,曾兴艳,聂奎,曹清安.川渝地区奶牛隐性乳房炎调查和主要病原菌分离鉴定及药敏试验[J].中兽医医药杂志,2010,29(3):16-19. |
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| 作者姓名： | 王裕文 曾兴艳 聂奎 曹清安 |
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| 作者单位： | 1. 西南大学动物科技学院,重庆,400715
2. 农标普瑞纳(重庆)饲料有限公司 |
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| 摘 要： | 用LMT法对川渝地区134头泌乳奶牛的491个乳区进行隐性乳房炎发病情况调查,并对阳性乳样进行主要病原菌的分离鉴定及药敏试验。结果显示,被检奶牛患隐性乳房炎头阳性率为70.90%,乳区阳性率为35.64%,175份阳性乳样的细菌检出率为90.86%,病原菌单独感染率为10.69%,混合感染率为89.31%;共分离到金黄色葡萄球菌、链球菌和大肠杆菌3种主要病原菌134株,其中金黄色葡萄球菌93株,占分离菌株的69.40%,链球菌34株,占25.37%,大肠杆菌7株,占5.23%;主要病原菌对呋喃妥因、新生霉素、头孢菌素类和喹诺酮类药物敏感性较好,对大多数抗菌药物产生不同程度的耐药性,对青霉素和链霉素则完全不敏感,耐药程度接近100%。
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| 关 键 词： | 奶牛 隐性乳房炎 主要病原菌 分离鉴定 药敏试验 |
Isolation, identification and drug sensitivity test of main pathogenic bacteria of bovine subclinical mastitis in Sichuan and Chongqing Area |
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| Institution: | WANG Yu-wen,ZENG Xing-yan,NIE Kui(College of Animal Science and Technology,Southwest University,Chongqing 400715,China) |
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| Abstract: | The epidemiological survey of subclinical mastitis of 491 mammary regions of 134 dairy cow in Sichuan and Chongqing were carried out by LMT,and main pathogenic bacteria were isolated from the positive milk samples and identified,then drug sensitivity test was performed.The results showed that the positive rate of bovine subclinical mastitis was 70.90%,and the positive rate of mammary region was 35.64%.The isolation rate of pathogenic bacteria was 90.86%（159/175）,10.69% of which were infected by only one kind of bacteria,while 89.31% were infected by more than one kind of bacteria.137 strains of bacteria were isolated from the 159 positive milk samples,93 strains among them were Staphylococcus aureus（69.40%）,34 were Streptococcus（25.37%）,and the others were E.coli（5.23%）.Drug sensitivity test showed that all pathogenic bacteria were resistant to Penicillin and Streptomycin,while Nitrofurantion,Novobiocin,Cephalosporins and Quinolones could obvious inhibit the growth of these main pathogenic bacteria. |
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| Keywords: | subclinical mastitis main pathogenic bacteria isolation and identification drug sensitivity test dairy cow |
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