| 接种猪瘟兔化弱毒株的永生化猪血管内皮细胞传代情况研究 |
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| 引用本文： | 谭晓妮,张彦明,张旭,邓力.接种猪瘟兔化弱毒株的永生化猪血管内皮细胞传代情况研究[J].动物医学进展,2010,31(7):11-15. |
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| 作者姓名： | 谭晓妮 张彦明 张旭 邓力 |
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| 作者单位： | 西北农林科技大学动物医学院,陕西杨陵,712100 |
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| 摘 要： | 在传至70代的永生化猪血管内皮细胞接种猪瘟兔化弱毒,72 h后收取细胞上清液并对处理的细胞进行传代培养,分别检测第70、75、80、90、100、105代接毒细胞中病毒,观察各代细胞的生长情况。结果表明,接种猪瘟兔化弱毒后20代(第90代永生化猪血管内皮细胞)的细胞与正常的形态相似,但传至接毒后35代(第105代永生化猪血管内皮细胞)时,细胞稍有拉长现象。各代次细胞中都有猪瘟兔化弱毒的检出。本研究为永生化猪血管内皮细胞生产猪瘟兔化弱毒疫苗奠定了基础。
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| 关 键 词： | 猪瘟兔化弱毒株(HCLV) 永生化猪血管内皮细胞 形态变化 细胞传代 |
Study on the Growth Characteristic of Immortalized Swine Vascular Endothelial Cells Infected with Lapinized Chinese Strain of Classical Swine Fever Virus |
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| TAN Xiao-ni,ZHANG Yan-ming,ZHANG Xu,DENG Li.Study on the Growth Characteristic of Immortalized Swine Vascular Endothelial Cells Infected with Lapinized Chinese Strain of Classical Swine Fever Virus[J].Progress In Veterinary Medicine,2010,31(7):11-15. |
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| Authors: | TAN Xiao-ni ZHANG Yan-ming ZHANG Xu DENG Li |
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| Institution: | TAN Xiao-ni,ZHANG Yan-ming,ZHANG Xu,DENG Li (College of Veterinary Medicine,Northwest A&F University,Yangling,Shaanxi,712100,China) |
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| Abstract: | The 70th immortalized swine vascular endothelial cells was inoculated with lapinized Chinese strain(c-strain) of classical swine fever virus(CSFV).The cultural supernatants were collected for virus detection and the infected cells were passaged culture in the 72 h after inoculation.The morphological character of infected cells and mock cells were observed and the HCLV was determined in the 70th,75th,80th,90th,100th and 105th generations infected cells.The results showed that the morphological character of i... |
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| Keywords: | HCLV strain immortalized swine vascular endothelial cells morphological change cell generation |
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