| 新城疫病毒山东强毒株的分离鉴定 |
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| 引用本文： | 沈志强,刘吉山,李峰,林初文,徐可利.新城疫病毒山东强毒株的分离鉴定[J].中国预防兽医学报,2002,24(2):130-132. |
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| 作者姓名： | 沈志强 刘吉山 李峰 林初文 徐可利 |
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| 作者单位： | 山东省滨州畜牧兽医研究所,山东省畜禽用蜂胶疫苗研究开发推广中心,山东,滨州,256618 |
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| 摘 要： | 从山东流行烈性传染病的鸡群中分离出7株具有血凝活性的病毒，该7株病毒的血凝活性均能被新城疫La Sota株标准阳性血清所抑制，但病毒不能被中和，仍能致死鸡胚。经分离鉴定，分离毒均为新成疫病毒株。通过鸡胚半致死量（ELD50）、最小致死量致鸡胚的平均时间（MDT）、1日龄雏鸡脑内致死指数（ICPI）、6周龄雏鸡静脉致死指数（IVPI）、血凝解脱及血凝素稳定性等试验表明：7株分离病毒均为新城疫病毒强毒性，其毒力与标准强毒株F48E9株相似。
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| 关 键 词： | 新城疫病毒 山东 强毒株 分离鉴定 NDV毒株 生物学特性 鸡 |
| 文章编号： | 1008-0589(2002)02-0130-03 |
| 修稿时间： | 2001年3月15日 |
Isolation and Identification of High Virulent Newcastle Disease Virus in Shandong Province |
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| SHEN Zhi-qiang,LIU Ji-shan,LI Feng,LIN Chu-wen,XU Ke-li.Isolation and Identification of High Virulent Newcastle Disease Virus in Shandong Province[J].Chinese Journal of Preventive Veterinary Medicine,2002,24(2):130-132. |
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| Authors: | SHEN Zhi-qiang LIU Ji-shan LI Feng LIN Chu-wen XU Ke-li |
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| Abstract: | isolates of viruses were isolated from 7 chicken flocks with high morbidity and mortality cotagious disease.These isolates are able to agglutinate chicken red blood cell which can be inhibited by Newcastle disease virus La Sota strain standard antisera,but cannot be neutralized by the antisera,they were still able to kill chicken embryos.These isolates were confirmed to be Newcastle disease virus based on 50% embryo lethal dose (ELD 50),mean death time (MDT) of chicken embryos,intracerebral pathogenicity index (ICPI) in 1-day-old chickens,intravenous pathogenicity index (IVPI) in 6-week-old chickens,dehaemagglutination and haemagglutinin heat-stability.The results demonstrated that these isolates were high virulent Newcastle disease virus,which were virulent as similar as F 48E 9 strain. |
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| Keywords: | Newcastle disease virus High virulent strain Isolation and identification |
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