| 应用噬菌体生物扩增技术检测牛结核分枝杆菌的方法研究 |
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| 引用本文： | 刘子芝,赵勇,刘纪艳,杨建华,尹望中,王颖,赵晶,周永燚.应用噬菌体生物扩增技术检测牛结核分枝杆菌的方法研究[J].中国动物检疫,2008,25(10):20-21. |
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| 作者姓名： | 刘子芝 赵勇 刘纪艳 杨建华 尹望中 王颖 赵晶 周永燚 |
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| 作者单位： | 天津市动物检疫站,300211 |
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| 摘 要： | 建立噬菌体生物扩增技术检测牛结核分枝杆菌试验方法,探讨最佳检测条件。比较各种检测方法对测定结果的影响。结果:噬菌体工作浓度为1×108PFU/ml、37℃感染2h,杀毒剂浓度在100mmol/L、室温作用10min为最佳检测条件。指示细胞浓度在1×106条/ml时,形成噬菌斑清晰,便于计数。结论:在选择最佳测定条件下,噬菌体生物扩增法检测牛结核分枝杆菌,快速、简便、灵敏。
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| 关 键 词： | 噬菌体 牛结核分枝杆菌 检测方法 |
Application of Bacteriophage Biological Amplification Technique for the Rapid Detection of Mycobacterium bovis |
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| LIU Zizhi,ZHAO Yong,LIU Jiyan,YANG Jianhu,YIN Wangzhong,WANG Ying,ZHAO Jing,ZHOU Yongyi.Application of Bacteriophage Biological Amplification Technique for the Rapid Detection of Mycobacterium bovis[J].China Journal Of Animal Quarantine,2008,25(10):20-21. |
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| Authors: | LIU Zizhi ZHAO Yong LIU Jiyan YANG Jianhu YIN Wangzhong WANG Ying ZHAO Jing ZHOU Yongyi |
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| Institution: | yi(Tianjin City Animal Quarantines Station 300211) |
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| Abstract: | Bacteriophage biological amplification technique was established for the detection of Mycobacterium bovis, the optimal test conditions were discussed.Result:the optimal test conditions were the working backerophage concentration I×108PFU/mL, infection at 37℃ for 2 hours and germicide(ferrous ammouium sulfate) concentration 100mmol/L at room temperature for 10 minutes.When the index cell concentration was 1 ×106/mL, the phage plagues were clear and easy to count.Conclusion:the bacteriophage bio-amplification method was rapid, simple and sensitive for the detection of Mycobacterium bovis under the selected optimal conditions. |
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| Keywords: | mycobacterium M bovis testing method |
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