| 不同血清型牛源巴氏杆菌多重PCR检测方法的建立 |
| |
| 引用本文： | 段新华,李建军,杨学云,王登峰,王治才.不同血清型牛源巴氏杆菌多重PCR检测方法的建立[J].中国畜牧兽医,2010,37(8):175-176. |
| |
| 作者姓名： | 段新华 李建军 杨学云 王登峰 王治才 |
| |
| 作者单位： | （新疆畜牧科学院兽医研究所，乌鲁木齐 830000） |
| |
| 基金项目： | 新疆维吾尔自治区十一五重大科技科技专项 |
| |
| 摘 要： | 巴氏杆菌是引起牛出血性败血症的主要病原之一,其致病血清型主要有荚膜A、B和E型。本试验选择、合成了针对3种不同血清型菌株的引物,建立了检测不同血清型菌株的多重PCR鉴别诊断方法。试验用2.5ngDNA模板即可扩增出目的基因,通过对引进的参考菌株进行检测表明,用该方法进行牛源巴氏杆菌的诊断和菌株分型特异性好,敏感性高。
|
| 关 键 词： | 巴氏杆菌 牛出血性败血症 多重PCR |
Multiplex PCR for Identification of Bovine Pasteurella mutocida from Different Capsular Serotype |
| |
| DUAN Xin-hua,LI Jian-jun,YANG Xue-yun,WANG Deng-feng,WANG Zhi-cai.Multiplex PCR for Identification of Bovine Pasteurella mutocida from Different Capsular Serotype[J].China Animal Husbandry & Veterinary Medicine,2010,37(8):175-176. |
| |
| Authors: | DUAN Xin-hua LI Jian-jun YANG Xue-yun WANG Deng-feng WANG Zhi-cai |
| |
| Institution: | (Institute of Veterinary Medicine, Xinjiang Academy of Animal Science, Urumqi 830000，China) |
| |
| Abstract: | Pasteurella mutocida is an important pathogen of bovine hemorrhagic septicemia and the pathogenic serotype are capsular serotype A, B and E. Three specific primers to capsular serotype A, B and E of Pasteurella mutocida were selected and synthesized and multiplex PCR method used for identification of different capsular serotype of Pasteurella mutocida was developed. Target genes could be amplified with 2.5 ng DNA template. The results showed that the method was specific, high sensitive in identification and classification of Pasteurella mutocida. |
| |
| Keywords: | Pasteurella mutocida bovine hemorrhagic septicemia multiplex PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
